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para-Aminothiophenol Radical Reaction-Functionalized Gold Nanoprobe for One-to-All Detection of Five Reactive Oxygen Species In Vivo
Kai Cui1,2; Chenchen Fan1,2; Gaoxian Chen1,2; Yuanyuan Qiu1,2; Mingwang Li1,2; Miao Lin1,2; Jian-Bo Wan5; Changsi Cai6; Zeyu Xiao1,2,3,4
2018-10
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume90Issue:20Pages:12137-12144
Abstract

Five major reactive oxygen species (ROS) are generated in diseases including H2O2, (OH)-O-center dot, O-2(center dot-), ROO center dot, and O-1(2). Simultaneous detection of the five ROS with a single probe is crucial for a comprehensive understanding of the development and progression of many diseases, such as cancer and inflammatory diseases. However, currently reported detection systems are limited by targeting one ROS with one probe. This one-to-one detection mode may fail to sufficiently unveil the diseased state. In this study, we achieved simultaneous detection of all the five ROS with one probe (i.e., one-to-all detection), by designing a novel para-aminothiophenol (PATP) and hemin-decorated gold (Au/PATP/Hemin) nanoprobe. The design is principled by our discovery that PATP can react with (OH)-O-center dot, O-2(center dot-), ROO center dot, and O-1(2) by a radical oxidative coupling mechanism to form 4,4'-dimercaptoazobenzene (DMAB). The DMAB then elicited strong characteristic surface-enhanced Raman scattering (SERS) peaks at 1142, 1386, and 1432 cm(-1); which in turn enables direct detection of (OH)-O-center dot, O-2(center dot-), ROO center dot, and O-1(2) and indirect detection of H2O2 by hemin-catalyzed fenton reaction to convert H2O2 into (OH)-O-center dot. In two representative ROS-elevated mice models of tumors and allergic dermatitis, the Au/PATP/Hemin nanoprobe demonstrated its robust performance of monitoring tumor development and inflammation progression in a highly sensitive and quantitative manner.

DOI10.1021/acs.analchem.8b03116
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000447816400055
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
2.Translational Medicine Collaborative Innovation Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China
3.Institute of Molecular Medicine, Ren Ji Hospital
4.Collaborative Innovation Center of Systems Biomedicine, Shanghai Jiao Tong University School of Medicine, Shanghai, China
5.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa,Macao, China
6.Center for Neuroscience, Faculty of Medicine and Health Science, University of Copenhagen, 2200 Copenhagen N, Denmark
Recommended Citation
GB/T 7714
Kai Cui,Chenchen Fan,Gaoxian Chen,et al. para-Aminothiophenol Radical Reaction-Functionalized Gold Nanoprobe for One-to-All Detection of Five Reactive Oxygen Species In Vivo[J]. ANALYTICAL CHEMISTRY,2018,90(20):12137-12144.
APA Kai Cui.,Chenchen Fan.,Gaoxian Chen.,Yuanyuan Qiu.,Mingwang Li.,...&Zeyu Xiao.(2018).para-Aminothiophenol Radical Reaction-Functionalized Gold Nanoprobe for One-to-All Detection of Five Reactive Oxygen Species In Vivo.ANALYTICAL CHEMISTRY,90(20),12137-12144.
MLA Kai Cui,et al."para-Aminothiophenol Radical Reaction-Functionalized Gold Nanoprobe for One-to-All Detection of Five Reactive Oxygen Species In Vivo".ANALYTICAL CHEMISTRY 90.20(2018):12137-12144.
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