UM  > 中華醫藥研究院
An extendable all-in-one injection twin derivatization LC-MS/MS strategy for the absolute quantification of multiple chemical-group-based submetabolomes
Yezhen Huang1; Yu Jiao1,2; Yiqiao Gao1; Pei Zhang1,3,4; Wanqiu Huang1; Yong Liu1,2; Yin Huang1; Yuan Tian1; Jian-Bo Wan5; Zunjian Zhang1; Kun Hao1,6; Fengguo Xu1
2019-07
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume1063Pages:99-109
Abstract

The ability of LC-MS/MS for high coverage metabolite analysis lags behind the requirements of global metabolomics. The introduction of chemical derivatizations could significantly extend the ability of LC-MS/MS with enhanced MS response and improved LC separation, which has been serving as a promising quantitative tool for metabolomic analysis. However, as one specific derivatization reagent usually targets to a certain moiety, only a single chemical-group-based submetabolome could be analyzed in one injection. Therefore, the coverage of detected metabolites by derivatization-based LC-MS/MS is largely limited. To overcome this technical obstacle of derivatization-based LC-MS and increase submetabolome coverage, we proposed an extendable all-in-one injection LC-MS/MS strategy. 5-dimethylamino-naphthalene-1-sulfonyl chloride (Dns-Cl)/5-diethylamino-naphthalene-1-sulfonyl chloride (Dens-Cl) and 5-dimethylamino-naphthalene-1-sulfonyl piperazine (Dns-PP)/5-diethylamino-naphthalene-1-sulfonyl piperazine (Dens-PP) were used as twins labeling reagents for amino/phenol and carboxyl submetabolomes, respectively. “Series Mode” and “Parallel Mode” were proposed and investigated using eight representative standards with the consideration of interaction between different derivatization systems, time-consumption, and extendability. As a result, we found that “Series Mode” led to yield reduction, while “Parallel Mode” gave identical results with those of individual derivatization. Finally, a “Parallel Mode” was chosen to develop an extendable all-in-one injection twin derivatization LC-MS/MS strategy to quantify eighty metabolites assigned to five classes of microbial metabolites, including polyamines, amino acids, indole derivatives, bile acids, and free fatty acids. This well-validated method quantified 67 metabolites absolutely and discovered additional 40 differential metabolites compared with the untargeted method in rat serum from irinotecan (CPT-11)-induced gastrointestinal toxicity model.

KeywordAll-in-one Injection Strategy Twins Derivatization Liquid Chromatography‒tandem Mass
DOI10.1016/j.aca.2019.02.001
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000463815800011
Fulltext Access
Citation statistics
Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing, 210009, PR China
2.Department of Organic Chemistry, China Pharmaceutical University, Nanjing, 210009, China
3.Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Japan
4.Division of Physiological Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
5.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China
6.Key Laboratory of Drug Metabolism&Pharmacokinetics, China Pharmaceutical University, Nanjing, 210009, China
Recommended Citation
GB/T 7714
Yezhen Huang,Yu Jiao,Yiqiao Gao,et al. An extendable all-in-one injection twin derivatization LC-MS/MS strategy for the absolute quantification of multiple chemical-group-based submetabolomes[J]. ANALYTICA CHIMICA ACTA,2019,1063:99-109.
APA Yezhen Huang.,Yu Jiao.,Yiqiao Gao.,Pei Zhang.,Wanqiu Huang.,...&Fengguo Xu.(2019).An extendable all-in-one injection twin derivatization LC-MS/MS strategy for the absolute quantification of multiple chemical-group-based submetabolomes.ANALYTICA CHIMICA ACTA,1063,99-109.
MLA Yezhen Huang,et al."An extendable all-in-one injection twin derivatization LC-MS/MS strategy for the absolute quantification of multiple chemical-group-based submetabolomes".ANALYTICA CHIMICA ACTA 1063(2019):99-109.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yezhen Huang]'s Articles
[Yu Jiao]'s Articles
[Yiqiao Gao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yezhen Huang]'s Articles
[Yu Jiao]'s Articles
[Yiqiao Gao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yezhen Huang]'s Articles
[Yu Jiao]'s Articles
[Yiqiao Gao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.