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Zebrafish: An in-vivo model to evaluate absorption and biodistribution of nanocrystals
Ye Li1; Xiaoqing Miao1; Liang Wang1; Xiang Yi2; Simon M.Y. Lee1; Ying Zheng1
2016-02
Source PublicationNanomedicine: Nanotechnology, Biology, and Medicine
ISSN1549-9634
Volume12Issue:2Pages:573
Other Abstract

Nanosized molecules possess enormous potential in imaging and therapeutic treatment. Due to their special physiochemical properties, their in-vivo behavior including absorption and biodistribution is worth to be studied. Zebrafish have emerged as useful vertebrate models for the optical tracking and biocompatibility assessment because of their transparent body and similar homology to human. This study aimed to explore the absorption and biodistribution of nanocrystals in zebrafish model. A highly lipophilic compound named coumarin-6 (C6) was selected and formulated as C6-nanocrystals (C6-NCs) by anti-solvent precipitation method with an average particle size of 67.5 ± 5.2 nm. Larval zebrafish at 8 days post-fertilization were incubated with different concentrations of C6-NCs.Whole body of zebrafish was collected from 5 to 60 min to take fluorescence images. C6 was also extracted and quantified by florescence detector. As shown in Figure 1, A, no obvious fluorescence was observed in C6 suspension treated group, while strong fluorescence was observed in C6-NCs treated group, and the intensity of fluorescence emission increased at dose dependent manner. Above result was consistent with the quantification result shown in Figure 1, B. The concentration of C6 increased from 5 min to 30 min and reached to a plateau at 60 min (Figure 1, C). After immersing for 30 min, C6-NCs were replaced by fresh medium. Zebrafish were collected and processed at different times up to 48 h. As shown in Figure 1, D, C6-NCs were significantly uptaken by zebrafish with clear regions of strong fluorescence in the eye, gall bladder, intestine and brain. C6-NCs were readily absorbed and distributed in zebrafish but eliminated very slowly. After 48 h, there was still a part of C6 accumulated in the zebrafish body, especially in the digestive system. These findings suggest that nanocrystals could enhance in-vivo absorption and biodistribution of C6 compared to the free drug. In conclusion, zebrafish could be developed as a useful in-vivo model for preliminary and rapid evaluation the absorption and biodistribution of the nanosized formulation.

DOIhttps://doi.org/10.1016/j.nano.2015.12.352
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics ; Research & Experimental Medicine
WOS SubjectNanoscience & Nanotechnology ; Medicine, Research & Experimental
WOS IDWOS:000373923400369
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYing Zheng
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau SAR, China
2.Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, USA
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Ye Li,Xiaoqing Miao,Liang Wang,et al. Zebrafish: An in-vivo model to evaluate absorption and biodistribution of nanocrystals[J]. Nanomedicine: Nanotechnology, Biology, and Medicine,2016,12(2):573.
APA Ye Li,Xiaoqing Miao,Liang Wang,Xiang Yi,Simon M.Y. Lee,&Ying Zheng.(2016).Zebrafish: An in-vivo model to evaluate absorption and biodistribution of nanocrystals.Nanomedicine: Nanotechnology, Biology, and Medicine,12(2),573.
MLA Ye Li,et al."Zebrafish: An in-vivo model to evaluate absorption and biodistribution of nanocrystals".Nanomedicine: Nanotechnology, Biology, and Medicine 12.2(2016):573.
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