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A novel non-viral gene vector for hepatocyte-targeting and in situmonitoring of DNA delivery in single cells
Yong-Guang Gao1; Quan Tang1; You-Di Shi1; Ying Zhang1; Ruibing Wang2; Zhong-Lin Lu1
2016
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume6Issue:55Pages:50053-50060
Abstract

A macrocyclic polyamine lipid bearing both fluorescent naphthalimide and targeting cholic acid moieties, 1, was designed and synthesized as a novel non-viral gene vector. Plasmid DNA was completely condensed at 10 mu M of 1 (N/P = 3.6) in 5 minutes at 37 degrees C. The process of DNA uptake process by 1 in a single cell was clearly investigated through time-dependent fluorescence microscopy assay. Flow cytometry experiments of liposome 1 clearly proved its targeting activity toward HepG2 cells. Luciferase gene expression transfected by the DNA complex of 1/DOPE gave a higher transfection efficiency in HepG2, A549, HeLa, U2OS and CCC-HPF-1 cells than by lipofectamine 2000. The present work demonstrated that the designed multifunctional organic molecule is able to fluorescently track the gene delivery process at a single-cell level for the first time. This will greatly aid mechanistic studies of gene transfection and further development of new gene vectors for biomedical applications.

DOI10.1039/c6ra08935f
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000377577800089
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhong-Lin Lu
Affiliation1.Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19,Beijing 100875, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, China
Recommended Citation
GB/T 7714
Yong-Guang Gao,Quan Tang,You-Di Shi,et al. A novel non-viral gene vector for hepatocyte-targeting and in situmonitoring of DNA delivery in single cells[J]. RSC ADVANCES,2016,6(55):50053-50060.
APA Yong-Guang Gao,Quan Tang,You-Di Shi,Ying Zhang,Ruibing Wang,&Zhong-Lin Lu.(2016).A novel non-viral gene vector for hepatocyte-targeting and in situmonitoring of DNA delivery in single cells.RSC ADVANCES,6(55),50053-50060.
MLA Yong-Guang Gao,et al."A novel non-viral gene vector for hepatocyte-targeting and in situmonitoring of DNA delivery in single cells".RSC ADVANCES 6.55(2016):50053-50060.
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