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Bio-assay guided identification of hepatoprotective polyphenols from Penthorum chinense Pursh on t-BHP induced oxidative stress injured L02 cells
Anqi Wang; Shengpeng Wang; Yun Jiang; Meiwan Chen; Yitao Wang; Ligen Lin
2016
Source PublicationFOOD & FUNCTION
ISSN2042-6496
Volume7Issue:4Pages:2074-2083
Abstract

Penthorum chinense Pursh (Ganhuangcao in Chinese) is traditionally used for liver protection and treatment of liver diseases, including hepatitis B, hepatitis C and alcoholic liver damage. We and others have disclosed the hepatoprotective effect of different extracts from P. chinense. To further explore the chemical principles responsible for its liver protective effect, a bioactivity guided isolation was carried out on the water extract of P. chinense, which led to the identification of an effective fraction (E50M60). Further isolation of the E50M60 fraction produced ten polyphenols. Among them, quercetin showed the most significant protective effect on tert-butyl hydroperoxide (t-BHP) induced hepatocyte damage. Further study demonstrated that both the E50M60 fraction and quercetin attenuated t-BHP induced hepatocyte apoptosis through up-regulating the expression of B-cell lymphoma-2 protein (BCL-2) and BCL-xL, and down-regulating the cleaved products of caspase-7, -9 and nuclear poly(ADP-ribose) polymerase (PARP). Moreover, the E50M60 fraction and quercetin promoted nuclear factor-like 2 (NRF2), superoxide dismutase-2 (SOD-2) and heme oxygenase-1 (HO-1) expressions and suppressed Kelch-like ECH-associated protein 1 (KEAP-1) expression, resulting in resistance to the reactive oxygen species (ROS) induced mitochondrial oxidative stress. Altogether, both the active fraction and quercetin from P. chinense might be well developed as a novel functional food for liver protection.

DOI10.1039/c6fo00110f
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Food Science & Technology
WOS SubjectBiochemistry & Molecular Biology ; Food Science & Technology
WOS IDWOS:000374790000040
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLigen Lin
AffiliationState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macau,China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Anqi Wang,Shengpeng Wang,Yun Jiang,et al. Bio-assay guided identification of hepatoprotective polyphenols from Penthorum chinense Pursh on t-BHP induced oxidative stress injured L02 cells[J]. FOOD & FUNCTION,2016,7(4):2074-2083.
APA Anqi Wang,Shengpeng Wang,Yun Jiang,Meiwan Chen,Yitao Wang,&Ligen Lin.(2016).Bio-assay guided identification of hepatoprotective polyphenols from Penthorum chinense Pursh on t-BHP induced oxidative stress injured L02 cells.FOOD & FUNCTION,7(4),2074-2083.
MLA Anqi Wang,et al."Bio-assay guided identification of hepatoprotective polyphenols from Penthorum chinense Pursh on t-BHP induced oxidative stress injured L02 cells".FOOD & FUNCTION 7.4(2016):2074-2083.
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