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Simultaneously enantiospecific determination of (+)-trans-khellactone, ( plus /-)-praeruptorin A, ( plus /-)-praeruptorin B, (+)-praeruptorin E, and their metabolites, ( plus /-)-cis-khellactone, in rat plasma using online solid phase extraction-chiral LC-MS/MS
Yuelin Songa1; Wanghui Jinga1; Fengqing Yang2; Zhan Shi3; Meicun Yao3; Ru Yan1; Yitao Wang1
2014
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
Volume0731-7085Pages:269-277
Abstract

Many chiral drugs are used as the racemic mixtures in clinical practice. The occurrence of enantioselectively pharmacological activities calls for the development of enantiospecific analytical approaches during pharmacokinetic studies of enantiomers. Sample preparation plays a key role during quantitative analysis of biological samples. In current study, a rapid and reliable online solid phase extraction–chiral high performance liquid chromatography–tandem mass spectrometry (online SPE–chiral LC–MS/MS) method was developed for the simultaneously enantiospecific quantitation of (+)-trans-khellactone (dTK), (+/−)-cis-khellactone (d/lCK), (+/−)-praeruptorin A (d/lPA), (+/−)-praeruptorin B (d/lPB) and (+)- praeruptorin E (dPE), the main active angular-type pyranocoumarins (APs) in Peucedani Radix (Chinese name: Qian-hu) or themajormetabolites of those APs, in rat plasma. The validation assay results described here show good selectivity and enantiospecificity, extraction efficiency, accuracy and precision with quantification limits (LOQs) of 2.57, 1.28, 1.28, 1.88, 4.16, 4.16 and 4.18 ng mL−1 for dTK, lCK, dCK, dPA, dPB, lPB and dPE, respectively, while lPA was not detected in rat plasma due to the carboxylesterase(s)- mediated hydrolysis. In addition, the validated system was satisfactorily applied to characterize the pharmacokinetic properties of those components in normal and chronic obstructive pulmonary disease (COPD) rats following oral administration of Qian-hu extract. dCK and lCK were observed as the main herb-related compounds in plasma. Enantioselectively pharmacokinetic profiles occurred for dCK vs lCK, dPA vs lPA, and dPB vs lPB in either normal or COPD rats. The proposed whole system is expected to be a preferable analytical tool for in vivo study of chiral drugs, in particular for the characterization of enantioselectively pharmacokinetic profiles.

KeywordPharmacokinetics Online Spe Chiral Lc–ms/ms Enantiospecific Peucedani Radix
DOI10.1016/j.jpba.2013.08.042
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000329532800037
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Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYitao Wang
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao
2.School of Chemistry and Chemical Engineering, Chongqing University, 401331 Chongqing, China
3.School of Pharmaceutical Sciences, Sun Yat-sen University, 510006 Guangzhou, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Yuelin Songa,Wanghui Jinga,Fengqing Yang,et al. Simultaneously enantiospecific determination of (+)-trans-khellactone, ( plus /-)-praeruptorin A, ( plus /-)-praeruptorin B, (+)-praeruptorin E, and their metabolites, ( plus /-)-cis-khellactone, in rat plasma using online solid phase extraction-chiral LC-MS/MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,0731-7085:269-277.
APA Yuelin Songa.,Wanghui Jinga.,Fengqing Yang.,Zhan Shi.,Meicun Yao.,...&Yitao Wang.(2014).Simultaneously enantiospecific determination of (+)-trans-khellactone, ( plus /-)-praeruptorin A, ( plus /-)-praeruptorin B, (+)-praeruptorin E, and their metabolites, ( plus /-)-cis-khellactone, in rat plasma using online solid phase extraction-chiral LC-MS/MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,0731-7085,269-277.
MLA Yuelin Songa,et al."Simultaneously enantiospecific determination of (+)-trans-khellactone, ( plus /-)-praeruptorin A, ( plus /-)-praeruptorin B, (+)-praeruptorin E, and their metabolites, ( plus /-)-cis-khellactone, in rat plasma using online solid phase extraction-chiral LC-MS/MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 0731-7085(2014):269-277.
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