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Assessment of candidate plant DNA barcodes using the Rutaceae family
LUO Kun1,2; CHEN ShiLin1; CHEN KeLi2; SONG JingYuan1; YAO Hui1; MA XinYe1; ZHU YingJie3; PANG XiaoHui1; YU Hua1; LI XiWen1,4; LIU Zhen2

DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention. Here, seven regions (psbA-trnH, matK, ycf5, rpoC1, rbcL, ITS2, and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae. To evaluate each barcode’s utility for species authentication, PCR amplification efficiency, genetic divergence, and barcoding gaps were assessed. We found that the ITS2 region exhibited the highest inter-specific divergence, and that this was significantly higher than the intra-specific variation in the “DNA barcoding gap” assessment and Wilcoxon two-sample tests. The ITS2 locus had the highest identification efficiency among all tested regions. In a previous study, we found that ITS2 was able to discriminate a wide range of plant taxa, and here we confirmed that ITS2 was also able to discriminate a number of closely related species. Therefore, we propose that ITS2 is a promising candidate barcode for plant species identification.

KeywordDna Barcoding Its2, Rutaceae Identification
Indexed BySCI
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000279506400009
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Cited Times [WOS]:38   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorCHEN ShiLin
Affiliation1.Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100193, China
2.College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430061, China
3.School of Bioscience and Engineering, Southwest Jiaotong University, Chengdu 610031, China
4.Department of Chemistry, Tsinghua University, Beijing 100084, China
Recommended Citation
GB/T 7714
LUO Kun,CHEN ShiLin,CHEN KeLi,et al. Assessment of candidate plant DNA barcodes using the Rutaceae family[J]. SCIENCE CHINA-LIFE SCIENCES,2010,53(6):701-708.
APA LUO Kun.,CHEN ShiLin.,CHEN KeLi.,SONG JingYuan.,YAO Hui.,...&LIU Zhen.(2010).Assessment of candidate plant DNA barcodes using the Rutaceae family.SCIENCE CHINA-LIFE SCIENCES,53(6),701-708.
MLA LUO Kun,et al."Assessment of candidate plant DNA barcodes using the Rutaceae family".SCIENCE CHINA-LIFE SCIENCES 53.6(2010):701-708.
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