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1,8-Cineole Ameliorates LPS-Induced Vascular Endothelium Dysfunction in Mice via PPAR-gamma Dependent Regulation of NF-kappa B
Ke-Gang Linghu1,2; Guo-Ping Wu1; Ling-Yun Fu1; Hong Yang1; Hai-Zhi Li1; Yan Chen1,3; Hua Yu2; Ling Tao3; Xiang-Chun Shen1
2019-03-07
Source PublicationFRONTIERS IN PHARMACOLOGY
ISSN1663-9812
Volume10
Abstract

1,8-Cineole (eucalyptol), a monoterpene, has been widely reported for the anti-inflammatory effects. Our previous data confirmed that 1,8-cineole ameliorated the inflammatory phenotype of human umbilical vein endothelial cells (HUVECs) by mediating NF-κB expression in vitro. At present, we investigated the protection effects of 1,8-cineole on vascular endothelium in lipopolysaccharide (LPS)-induced acute inflammatory injury mice and the potential mechanisms involved in the protection in HUVECs. Results from enzyme linked immunosorbent assays revealed that 1,8-cineole suppressed the secretion of interleukin (IL)-6 and IL-8 and increased the expression of IL-10 in the serum of LPS-induced mice. 1,8-Cineole reduced the inflammatory infiltration and the expression of vascular cell adhesion molecular 1 (VCAM-1) in the sections of thoracic aorta in LPS-induced acute inflammatory mice. Western blotting indicated that 1,8-cineole significantly decreased the phosphorylation of NF-κB p65 and increased the expression of PPAR-γ in the thoracic aorta tissue. 1,8-Cineole increased the expression of PPAR-γ in LPS-induced HUVECs. 1,8-Cineole and rosiglitazone reduced the protein and mRNA levels of VCAM-1, E-selectin, IL-6, and IL-8 in LPS-induced HUVECs, which could be reversed by the action of GW9662 (inhibitor of PPAR-γ). 1,8-Cineole and rosiglitazone blocked the LPS-induced IκBα degradation and NF-κB p65 nucleus translocation, which could be reversed by the pretreatment of GW9662 or silence of PPAR-γ gene. In conclusion, 1,8-cineole attenuated LPS-induced vascular endothelial cells injury via PPAR-γ dependent modulation of NF-κB.

Keyword1,8-cineole Human Umbilical Vein Endothelial Cell Lipopolysaccharide Nf-κb Ppar-γ
DOI10.3389/fphar.2019.00178
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000460777900001
PublisherFRONTIERS MEDIA SA, AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLing Tao; Xiang-Chun Shen
Affiliation1.The Department of Pharmacology of Materia Medica ,School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, China
2.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macau, China
3.The Department of Pharmaceutics of TCM (the High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability, the Union Key Laboratory of Guiyang City-Guizhou Medical University), School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Ke-Gang Linghu,Guo-Ping Wu,Ling-Yun Fu,et al. 1,8-Cineole Ameliorates LPS-Induced Vascular Endothelium Dysfunction in Mice via PPAR-gamma Dependent Regulation of NF-kappa B[J]. FRONTIERS IN PHARMACOLOGY,2019,10.
APA Ke-Gang Linghu.,Guo-Ping Wu.,Ling-Yun Fu.,Hong Yang.,Hai-Zhi Li.,...&Xiang-Chun Shen.(2019).1,8-Cineole Ameliorates LPS-Induced Vascular Endothelium Dysfunction in Mice via PPAR-gamma Dependent Regulation of NF-kappa B.FRONTIERS IN PHARMACOLOGY,10.
MLA Ke-Gang Linghu,et al."1,8-Cineole Ameliorates LPS-Induced Vascular Endothelium Dysfunction in Mice via PPAR-gamma Dependent Regulation of NF-kappa B".FRONTIERS IN PHARMACOLOGY 10(2019).
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