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New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes
Nikita I. Ershov1; Viatcheslav A. Mordvinov1; Egor B. Prokhortchouk3,7; Mariya Y. Pakharukova1,2; Konstantin V. Gunbin1; Kirill Ustyantsev1; Mikhail A. Genaev1; Alexander G. Blinov1; Alexander Mazur3; Eugenia Boulygina6; Svetlana Tsygankova6; Ekaterina Khrameeva7; Nikolay Chekanov4; Guangyi Fan4,5; An Xiao4; He Zhang4; Xun Xu4; Huanming Yang4; Victor Solovyev8; Simon Ming-Yuen Lee5; Xin Liu4; Dmitry A. Afonnikov1,2; Konstantin G. Skryabin3,6
2019-05
Source PublicationBMC GENOMICS
ISSN1471-2164
Abstract

Background

The three epidemiologically important Opisthorchiidae liver flukes Opisthorchis felineus, O. viverrini, and Clonorchis sinensis, are believed to harbour similar potencies to provoke hepatobiliary diseases in their definitive hosts, although their populations have substantially different ecogeographical aspects including habitat, preferred hosts, population structure. Lack of O. felineus genomic data is an obstacle to the development of comparative molecular biological approaches necessary to obtain new knowledge about the biology of Opisthorchiidae trematodes, to identify essential pathways linked to parasite-host interaction, to predict genes that contribute to liver fluke pathogenesis and for the effective prevention and control of the disease.

Results

Here we present the first draft genome assembly of O. felineus and its gene repertoire accompanied by a comparative analysis with that of O. viverrini and Clonorchis sinensis. We observed both noticeably high heterozygosity of the sequenced individual and substantial genetic diversity in a pooled sample. This indicates that potency of O. felineus population for rapid adaptive response to control and preventive measures of opisthorchiasis is higher than in O. viverrini and C. sinensis. We also have found that all three species are characterized by more intensive involvement of trans-splicing in RNA processing compared to other trematodes.

Conclusion

All revealed peculiarities of structural organization of genomes are of extreme importance for a proper description of genes and their products in these parasitic species. This should be taken into account both in academic and applied research of epidemiologically important liver flukes. Further comparative genomics studies of liver flukes and non-carcinogenic flatworms allow for generation of well-grounded hypotheses on the mechanisms underlying development of cholangiocarcinoma associated with opisthorchiasis and clonorchiasis as well as species-specific mechanisms of these diseases.

 

 
KeywordOpisthorchiidae Opisthorchis Felineus Genome Trans-splicing Microintrons Liver Flukes Transcriptome Metacercariae
DOI10.1186/s12864-019-5752-8
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000468781800006
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被引频次[WOS]:1   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Affiliation1.Institute of Cytology and Genetics SB RAS, 10 Lavrentiev Ave, Novosibirsk630090, Russia.2Novosibirsk State University
2.Pirogova Str, Novosibirsk630090, Russia
3.Russian Federal Research Center for Biotechnology, 33/2Leninsky prospect, Moscow 119071, Russia
4.BGI-Shenzhen, 11 BeishanIndustrial Zone, Yantian District, Shenzhen 518083, China
5.State KeyLaboratory of Quality Research in Chinese Medicine, Institute of ChineseMedical Sciences, University of Macau, Macao, China
6.Federal ResearchCenter Kurchatov Institute, Moscow, Russia
7.ZAO Genoanalytica, 1 LeninskieGory street, Moscow 119234, Russia
8.Softberry Inc., 116 Radio Circle, Suite400, Mount Kisco, NY 10549, USA
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Nikita I. Ershov,Viatcheslav A. Mordvinov,Egor B. Prokhortchouk,et al. New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes[J]. BMC GENOMICS,2019.
APA Nikita I. Ershov.,Viatcheslav A. Mordvinov.,Egor B. Prokhortchouk.,Mariya Y. Pakharukova.,Konstantin V. Gunbin.,...&Konstantin G. Skryabin.(2019).New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes.BMC GENOMICS.
MLA Nikita I. Ershov,et al."New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes".BMC GENOMICS (2019).
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