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Combined transcriptomic and proteomic analysis reveals a diversity of venom-related and toxin-like peptides expressed in the mat anemone Zoanthus natalensis (Cnidaria, Hexacorallia)
Qiwen Liao1; Guiyi Gong1; Terence C. W. Poon2; Irene L. Ang2; Kate M. K. Lei2; Shirley Weng In Siu3; Clarence Tsun Ting Wong4; Gandhi Rádis-Baptista5; Simon Ming-Yuen Lee1
2019-06
Source PublicationARCHIVES OF TOXICOLOGY
ISSN0340-5761
Volume93Issue:6Pages:1745-1767
Abstract

Venoms from marine animals have been recognized as a new emerging source of peptide-based therapeutics. Several peptide toxins from sea anemone have been investigated as therapeutic leads or pharmacological tools. Venom complexity should be further highlighted using combined strategies of large-scale sequencing and data analysis which integrated transcriptomics and proteomics to elucidate new proteins or peptides to be compared among species. In this work, transcriptomic and proteomic analyses were combined to identify six groups of expressed peptide toxins in Zoanthus natalensis. These include neurotoxin, hemostatic and hemorrhagic toxin, protease inhibitor, mixed function enzymes, venom auxiliary proteins, allergen peptides, and peptides related to the innate immunity. Molecular docking analysis indicated that one expressed Zoanthus Kunitz-like peptide, ZoaKuz1, could be a voltage-gated potassium channels blocker and, hence, it was selected for functional studies. Functional bioassays revealed that ZoaKuz1 has an intrinsic neuroprotective activity in zebrafish model of Parkinson’s disease. Since pharmacological blockade of KV channels is known to induce neuroprotective effects, ZoaKuz1 holds the potential to be developed in a therapeutic tool to control neural dysfunction by slowing or even halting neurodegeneration mediated by ion-channel hyperactivity.

KeywordCnidaria Neuroprotection Proteomic Transcriptomic Venom-derived Peptide Zoantharian
DOI10.1007/s00204-019-02456-z
Indexed BySCI
Language英语
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000475702100021
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Document TypeJournal article
专题Institute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, China
2.Pilot Laboratory and Proteomics Core, Faculty of Health Sciences, University of Macau, Macau, China
3.Department of Computer and Information Science, Faculty of Science and Technology, University of Macau, Macau, China
4.Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
5.Laboratory of Biochemistry and Biotechnology, Institute for Marine Sciences, Federal University of Ceará, Fortaleza, Brazil
First Author AffilicationInstitute of Chinese Medical Sciences
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Qiwen Liao,Guiyi Gong,Terence C. W. Poon,et al. Combined transcriptomic and proteomic analysis reveals a diversity of venom-related and toxin-like peptides expressed in the mat anemone Zoanthus natalensis (Cnidaria, Hexacorallia)[J]. ARCHIVES OF TOXICOLOGY,2019,93(6):1745-1767.
APA Qiwen Liao.,Guiyi Gong.,Terence C. W. Poon.,Irene L. Ang.,Kate M. K. Lei.,...&Simon Ming-Yuen Lee.(2019).Combined transcriptomic and proteomic analysis reveals a diversity of venom-related and toxin-like peptides expressed in the mat anemone Zoanthus natalensis (Cnidaria, Hexacorallia).ARCHIVES OF TOXICOLOGY,93(6),1745-1767.
MLA Qiwen Liao,et al."Combined transcriptomic and proteomic analysis reveals a diversity of venom-related and toxin-like peptides expressed in the mat anemone Zoanthus natalensis (Cnidaria, Hexacorallia)".ARCHIVES OF TOXICOLOGY 93.6(2019):1745-1767.
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