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In Vitro and In Vivo Anti-Inflammatory Effects of Polyphyllin VII through Downregulating MAPK and NF-kappa B PathwaysDownregulating MAPK and NF-kappa B Pathways
Zhang, C1,2; Li, CY3; Jia, XJ2; Wang, K2; Tu, YB2; Wang, RC4; Liu, KC4; Lu, T1; He, CW2
2019
Source PublicationMOLECULES
ISSN1420-3049
Volume24Issue:5
Abstract

Background: Polyphyllin VII (PP7), a steroidal saponin from Paris polyphylla, has been found to exert strong anticancer activity. Little is known about the anti-inflammatory property of PP7. In this study, the anti-inflammatory activity and its underlying mechanisms of PP7 were evaluated in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and in multiple animal models. Methods: The content of nitric oxide (NO) was determined by spectrophotometry. The levels of prostaglandin E2 (PGE2) and cytokines were measured by enzyme-linked immunosorbent assay (ELISA) assay. The mRNA expression of pro-inflammatory genes was determined by qPCR. The total and phosphorylated protein levels were examined by Western blotting. The in vivo anti-inflammatory activities were evaluated by using mouse and zebrafish models. Results: PP7 reduced the production of NO and PGE2 and the protein and mRNA expressions of pro-inflammatory cytokines (TNF-αIL-1β, and IL-6) and enzymes (inducible NO synthase [iNOS], cyclooxygenase-2 [COX-2], and Matrix metalloproteinase-9 [MMP-9]) in LPS-induced RAW264.7 cells by suppressing the NF-κB and MAPKs pathways. Notably, PP7 markedly inhibited xylene-induced ear edema and cotton pellet-induced granuloma formation in mice and suppressed LPS and CuSO4-induced inflammation and toxicity in zebrafish embryos. Conclusion: This study demonstrates that PP7 exerts strong anti-inflammatory activities in multiple in vitro and in vivo models and suggests that PP7 is a potential novel therapeutic agent for inflammatory diseases.

KeywordZebrafish Polyphyllin Vii Anti-inflammation Macrophage Mice
DOI10.3390/molecules24050875
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000462662900051
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorHe, CW
Affiliation1.School of Life Sciences, Beijing University of Chinese Medicine
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences,University of Macau
3.School of Pharmaceutical Sciences, Changchun University of Chinese Medicine
4.Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong ProvincialKey Laboratory for Biosensor, Biology Institute of Shandong Academy of Sciences
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Zhang, C,Li, CY,Jia, XJ,et al. In Vitro and In Vivo Anti-Inflammatory Effects of Polyphyllin VII through Downregulating MAPK and NF-kappa B PathwaysDownregulating MAPK and NF-kappa B Pathways[J]. MOLECULES,2019,24(5).
APA Zhang, C.,Li, CY.,Jia, XJ.,Wang, K.,Tu, YB.,...&He, CW.(2019).In Vitro and In Vivo Anti-Inflammatory Effects of Polyphyllin VII through Downregulating MAPK and NF-kappa B PathwaysDownregulating MAPK and NF-kappa B Pathways.MOLECULES,24(5).
MLA Zhang, C,et al."In Vitro and In Vivo Anti-Inflammatory Effects of Polyphyllin VII through Downregulating MAPK and NF-kappa B PathwaysDownregulating MAPK and NF-kappa B Pathways".MOLECULES 24.5(2019).
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