UM  > 中華醫藥研究院
Mitochondrial protective effect of neferine through the modulation of nuclear factor erythroid 2-related factor 2 signalling in ischaemic stroke
Chuanhong Wu1; Jianxin Chen3; Ruocong Yang3; Feipeng Duan3; Shaojing Li2; Xiuping Chen1
2019-02
Source PublicationBRITISH JOURNAL OF PHARMACOLOGY
ISSN0007-1188
Volume176Issue:3Pages:400-415
Abstract

BACKGROUND AND PURPOSE

Ischaemic stroke is a leading cause of death and long-term disability. Promising neuroprotective compounds are urgently needed to overcome clinical therapeutic limitations. Neuroprotective agents are limited to single-target agents, which further limit theirclinical effectiveness. Due to the brain’s particular energy requirements, the energy micro-environment, centred in mitochondria,is a new research hotspot in the complex pathology of ischaemic stroke. Here, we studied the effects of neferine (Nef), a bis-benzylisoquinoline alkaloid extracted from the seed embryo of Nelumbo nuciferaGaertn, on ischaemic stroke and its underlyingmitochondrial protective mechanisms.

EXPERIMENTAL APPROACH

Rats with permanent middle cerebral artery occlusion (pMCAO)-induced focal cerebral ischaemia andtert-butyl hydroperoxide(t-BHP)-injured PC12 cells were used to investigate the neuroprotective effects of Nef, particularly with regard to energymicro-environment regulation by mitochondria and its mechanismin vivoandin vitro.

KEY RESULTS

Nef protected t-BHP-injured PC12 cellsin vitro and ameliorated neurological score, infarct volume, regional cerebral blood flow,cerebral microstructure and oxidant-related enzyme deficits in pMCAO rats in vivo. Nef also prevented mitochondrial dysfunction both in vivo and in vitro. The underlying mechanism of the mitochondrial protective effect of Nef might be attributed to theincreased translocation of Nrf2 to the nucleus. Furthermore, the translocation of Nrf2 to nucleus was also decreased bysequestosome 1 (p62) knockdown.

CONCLUSIONS AND IMPLICATIONS

Our results demonstrated that Nef might have therapeutic potential for ischaemic stroke and may exert its protective role throughmitochondrial protection. This protection might be attributed to the modulation of Nrf2 signalling.

DOI10.1111/bph.14537
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000455517100005
PublisherWILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Fulltext Access
Citation statistics
Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorShaojing Li; Xiuping Chen
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Instituteof Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China
3.Beijing University of Chinese Medicine, Beijing, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chuanhong Wu,Jianxin Chen,Ruocong Yang,et al. Mitochondrial protective effect of neferine through the modulation of nuclear factor erythroid 2-related factor 2 signalling in ischaemic stroke[J]. BRITISH JOURNAL OF PHARMACOLOGY,2019,176(3):400-415.
APA Chuanhong Wu,Jianxin Chen,Ruocong Yang,Feipeng Duan,Shaojing Li,&Xiuping Chen.(2019).Mitochondrial protective effect of neferine through the modulation of nuclear factor erythroid 2-related factor 2 signalling in ischaemic stroke.BRITISH JOURNAL OF PHARMACOLOGY,176(3),400-415.
MLA Chuanhong Wu,et al."Mitochondrial protective effect of neferine through the modulation of nuclear factor erythroid 2-related factor 2 signalling in ischaemic stroke".BRITISH JOURNAL OF PHARMACOLOGY 176.3(2019):400-415.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chuanhong Wu]'s Articles
[Jianxin Chen]'s Articles
[Ruocong Yang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chuanhong Wu]'s Articles
[Jianxin Chen]'s Articles
[Ruocong Yang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chuanhong Wu]'s Articles
[Jianxin Chen]'s Articles
[Ruocong Yang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.