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Dual pH and oxygen luminescent nanoprobes based on graft polymers for extracellular metabolism monitoring and intracellular imaging
Tingting Pan1,2; Cheng Yang2; Jiayan Shi2; Changxiang Hao2; Yuan Qiao2; Jiaze Li2; Mengyu Deng2; Yanqing Tian2; Meiwan Chen1
2019-07
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume291Pages:306-318
Abstract

Cellular pH and oxygen are the two important parameters to evaluate cell metabolism and functions of subcellular structures. Herein, we developed graft copolymers-based dual pH and oxygen luminescent nanoprobes with excellent sensitivity and quantum yields for both extracellular sensing and intracellular imaging. The oxygen probe platinum(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (PtTFPP) was encapsulated into the hydrophobic core formed by poly(ε-caprolactone) (PCL), while pH sensitive fluorescein moieties were polymerized in hydrophilic chains of poly(oligo(ethylene glycol) methacrylate) (POEGMA). The quantum yield of oxygen and pH probe in the nanoprobes was separately achieved to be ˜0.15 under nitrogen and ˜0.78 at pH 8. Through tuning the polymer structures, the nanoprobes without the [2-(methacryloyloxy)ethyl] trimethylammonium chloride (MATMA) components (M1) was demonstrated to be extracellular probes; while the other nanoprobes with the MATMA moieties (M2) was demonstrated to be intracellular probe. Both nanostructured probes (<150 nm) exhibited fast, highly sensitive and reproducible responses to pH and dissolved oxygen (DO). Clear cell density dependent extracellular acidification and oxygen consumption were observed by using M1 during the growth of macrophages and E.coli. Whereas, M2 showed the ability for analysis of pH and DO in the macrophage cells through bioimaging. Clear emission intensity changes under confocal were observed between hypoxia and normoxia conditions as well as between pH 4.5 and 7.5, suggesting that these nanoprobes are capable of cell metabolism monitoring and intracellular pH and DO analysis. This study provided a feasible way to design extracellular or intracellular multi-parameters sensing systems, simultaneously with excellent optical and sensing properties for biological applications.

KeywordDual Ph And Oxygen Probes Amphiphilic Graft Copolymers Nanoprobes Extracellular Sensing Intracellular Imaging
DOI10.1016/j.snb.2019.04.082
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000467699900038
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macau, China
2.Department of Materials Science and Engineering, Southern University of Science and Technology, No 1088 Xueyuan Blvd, Xili, Nanshan District, Shenzhen, Guangdong,518055, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Tingting Pan,Cheng Yang,Jiayan Shi,et al. Dual pH and oxygen luminescent nanoprobes based on graft polymers for extracellular metabolism monitoring and intracellular imaging[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,291:306-318.
APA Tingting Pan.,Cheng Yang.,Jiayan Shi.,Changxiang Hao.,Yuan Qiao.,...&Meiwan Chen.(2019).Dual pH and oxygen luminescent nanoprobes based on graft polymers for extracellular metabolism monitoring and intracellular imaging.SENSORS AND ACTUATORS B-CHEMICAL,291,306-318.
MLA Tingting Pan,et al."Dual pH and oxygen luminescent nanoprobes based on graft polymers for extracellular metabolism monitoring and intracellular imaging".SENSORS AND ACTUATORS B-CHEMICAL 291(2019):306-318.
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