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Simultaneous Determination of Six Saponins in Panacis Japonici Rhizoma Using Quantitative Analysis of Multi-Components with Single-Marker Method
Wu, Qiu-Shuang1; Wang, Chun-Ming1; Lu, Jin-Jian1; Lin, Li-Gen1; Chen, Ping2; Zhang, Qing-Wen1
2017
Source PublicationCURRENT PHARMACEUTICAL ANALYSIS
ISSN1573-4129
Volume13Issue:3Pages:289-295
Abstract

Background: As the Panacis Japonici Rhizoma contains high content of saponins and most of the major saponins in this herb contain 2 to 4 units of saccharide, which made them very difficult to be purified in normal or reversed phase column chromatography.

 

Method: In this study, all analyses were performed on an Agilent 1200 series HPLC instrument and a Waters 2695 liquid chromatography. Both an Agilent Eclipse XDB-C18 column (4.6 mmx150 mm, 5μm) and an Agilent Eclipse Plus C18 column (4.6 mmx150 mm, 5 μm) were used. The mobile phase consisted of water containing 0.05% trifluoroacetic acid (pH 2.3) (A) and acetonitrile (B). The saponins were monitored at 203 nm.

 

Result: Chikusetsusaponin IVa was used as the internal constituent to calculate the relative correction factors (RCF) with other five constituents, respectively. The results showed that RCFs of all compounds with good reproducibility (RSD<5.0%) were obtained on different chromatography instruments. The content of the other five constituents was calculated with the RCF and the external standard (ES) method, respectively. Comparing the results obtained by these two methods, the QAMS is found to have no statistically significant difference as verified by the relative error < 2% and p > 0.05 of the paired t-test.

 

Conclusion: The QAMS method established in this research for simultaneous determination of the six triterpenoid saponins including ginsenoside Rg1, ginsenoside Rb1, chikusetsusaponin V, pseudoginsenoside RT1, chikusetsusaponin IV and chikusetsusaponin IVa is simple and feasible to evaluate the quality of Panacis Japonici Rhizoma.

KeywordPA.A. JA.oniC.s C. A. Meyer Panacis Japonici Rhizoma Qasm Triterpenoid Saponins Quantification
DOI10.2174/1573412912666160526165500
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000402460300012
PublisherBENTHAM SCIENCE PUBL LTD
The Source to ArticleWOS
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhang, Qing-Wen
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.College of Biology and Pharmaceutical engineering, Wuhan Polytechnic University, Wuhan 430023, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wu, Qiu-Shuang,Wang, Chun-Ming,Lu, Jin-Jian,et al. Simultaneous Determination of Six Saponins in Panacis Japonici Rhizoma Using Quantitative Analysis of Multi-Components with Single-Marker Method[J]. CURRENT PHARMACEUTICAL ANALYSIS,2017,13(3):289-295.
APA Wu, Qiu-Shuang,Wang, Chun-Ming,Lu, Jin-Jian,Lin, Li-Gen,Chen, Ping,&Zhang, Qing-Wen.(2017).Simultaneous Determination of Six Saponins in Panacis Japonici Rhizoma Using Quantitative Analysis of Multi-Components with Single-Marker Method.CURRENT PHARMACEUTICAL ANALYSIS,13(3),289-295.
MLA Wu, Qiu-Shuang,et al."Simultaneous Determination of Six Saponins in Panacis Japonici Rhizoma Using Quantitative Analysis of Multi-Components with Single-Marker Method".CURRENT PHARMACEUTICAL ANALYSIS 13.3(2017):289-295.
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