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Inhibition of Three Selected Beverage Extracts on alpha-Glucosidase and Rapid Identification of Their Active Compounds Using HPLC-DAD-MS/MS and Biochemical Detection
DE-QIANG LI; ZHENG-MING QIAN; SHAO-PING LI
2010
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
Volume58Issue:11Pages:6608-6613
Abstract

Inhibition of alpha-glucosidase is a therapeutic approach for diabetes. In this study, a method based on online liquid chromatography diode array detection tandem mass spectrometry and biochemical detection (LC-DAD-MS/MS-BCD) was developed to screen and identify alpha-glucosidase inhibitors from selected beverage extracts, including pu-erh tea (Camellia sinensis var. assamica), eagle tea (Litsea coreana Levi.), and radix glycyrrhizae (Glycyrrhiza uralensis Fisch.). As a result, two components, (-)-epigallocatechingallate (EGCG) and (-)-epicatechingallate (ECG), as potent alpha-glucosidase inhibitors, were found in pu-erh tea. The 1050 values of EGCG and ECG on alpha-glucosidase (EC 3.2.1.20, from Saccharomyces cerevisiae) were 175.1 and 246.9 mu M, respectively, and both were lower than that of acarbose (IC50 = 3553.0 mu M), a commercial alpha-glucosidase inhibitor. Kinetic studies revealed that both EGCG and ECG inhibited alpha-glucosidase activity in a noncompetitive manner. The study suggests that the developed LC-DAD-MS/MS-BCD system is a powerful tool for rapid screening and identification of alpha-glucosidase inhibitors in complex samples and that EGCG and ECG may be good candidates as alpha-glucosidase inhibitors.

KeywordPu-erh Tea Biochemical Detection Eagle Tea Radix Glycyrrhizae R-glucosidase Hplc
DOI10.1021/jf100853c
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000278149500010
PublisherAMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
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Cited Times [WOS]:61   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorSHAO-PING LI
AffiliationInstitute of Chinese Medical Sciences, University of Macau, Macao SAR, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
DE-QIANG LI,ZHENG-MING QIAN,SHAO-PING LI. Inhibition of Three Selected Beverage Extracts on alpha-Glucosidase and Rapid Identification of Their Active Compounds Using HPLC-DAD-MS/MS and Biochemical Detection[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2010,58(11):6608-6613.
APA DE-QIANG LI,ZHENG-MING QIAN,&SHAO-PING LI.(2010).Inhibition of Three Selected Beverage Extracts on alpha-Glucosidase and Rapid Identification of Their Active Compounds Using HPLC-DAD-MS/MS and Biochemical Detection.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,58(11),6608-6613.
MLA DE-QIANG LI,et al."Inhibition of Three Selected Beverage Extracts on alpha-Glucosidase and Rapid Identification of Their Active Compounds Using HPLC-DAD-MS/MS and Biochemical Detection".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 58.11(2010):6608-6613.
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