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Chronic MeHg exposure modifies the histone H3K4me3 epigenetic landscape in Caenorhabditis elegans
Rudgalvyte, Martina1,2; Peltonen, Juhani1; Lakso, Merja1; Wong, Garry2
2017-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN1532-0456
Volume191Pages:109-116
Abstract

Methylmercury (MeHg) is a persistent environmental pollutant that occurs in the food chain, at occupational sites, and via medical procedures. Exposure in humans and animal models results in renal, neuro, and reproductive toxicities. In this study, we demonstrate that chronic exposure to MeHg (10 jaM) causes epigenetic landscape modifications of histone H3K4 trimethylation (H3K4me3) marks in Caenorhabditis elegans using chromatin immuno-precipitation sequencing (ChIP-seq). The modifications correspond to the locations of 1467 genes with enhanced and 508 genes with reduced signals. Among enhanced genes are those encoding glutathione-S-transferases, lipocalin-related protein and a cuticular collagen. ChIP-seq enhancement of these genes was confirmed with increased mRNA expression levels revealed by qRT-PCR. Furthermore, we observed enhancement of H3K4me3 marks in these genes in animals exposed to MeHg in utero and assayed at L4 stage. In utero exposure enhanced marks without alterations in mRNA expression except for the 1pr-5 gene. Finally, knockdown of lipocalin-related protein gene 1pr-5, which is involved in intercellular signaling, and cuticular collagen gene dpy-7, structural component of the cuticle, by RNA interference (RNAi) resulted in increased lethality of animals after MeHg exposure. Our results provide new data on the epigenetic landscape changes elicited by MeHg exposure, as well as describe a unique model for studying in utero effects of heavy metals. Together, these findings may help to understand the toxicological effects of MeHg at the molecular level. (C) 2016 The Authors. Published by Elsevier Inc.

KeywordChromatin Immunoprecipitation Sequencing (Chip-seq) Rna Sequencing (Rna-seq) Heavy Metal Epigenetics Mercury
DOI10.1016/j.cbpc.2016.10.001
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS IDWOS:000391648000011
PublisherELSEVIER SCIENCE INC
The Source to ArticleWOS
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorWong, Garry
Affiliation1.A. I. Virtanen Institute for Molecular Sciences, Department of Neurobiology, University of Eastern Finland, Kuopio, Finland
2.Faculty of Health Sciences, University of Macau, Macau, S.A.R., China
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Rudgalvyte, Martina,Peltonen, Juhani,Lakso, Merja,et al. Chronic MeHg exposure modifies the histone H3K4me3 epigenetic landscape in Caenorhabditis elegans[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2017,191:109-116.
APA Rudgalvyte, Martina,Peltonen, Juhani,Lakso, Merja,&Wong, Garry.(2017).Chronic MeHg exposure modifies the histone H3K4me3 epigenetic landscape in Caenorhabditis elegans.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,191,109-116.
MLA Rudgalvyte, Martina,et al."Chronic MeHg exposure modifies the histone H3K4me3 epigenetic landscape in Caenorhabditis elegans".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 191(2017):109-116.
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