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Cupric ion functionalized polydopamine coated magnetic microspheres as solid-phase adsorbent for the extraction of purines in plasma
Dong-Dong Zhou1; Qian Zhang1; Hao Zhang1; Yin-Zhen Wang1; Feng-Qing Yang1; Sheng-Peng Wang2; Yi-Tao Wang2
2019-05-05
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1120Pages:95-103
Abstract

In this paper, Cu2+ functionalized Fe3O4@polydopamine core-shell (Fe3O4@PDA@Cu2+) magnetic microspheres were prepared by the chelation between Cu2+ and catechol of polydopamine surface. The synthetic magnetic adsorbent was characterized by Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, vibrating sample magnetometry, thermogravimetric analysis, scanning electron microscope and transmission electron microscopy. Four purines include guanine, adenine, hypoxanthine and xanthine were selected as model compounds to evaluate the applicability of this adsorbent. Several parameters that effected the extraction efficiency, such as extraction time, adsorbent amount, solution pH, ionic strength, eluent type, concentration of eluent and eluent time, were investigated. Under the optimized conditions, good linearity was obtained with correlation coefficients between 0.9983 and 0.9999 for the four analytes, and their LOD and LOQ were 0.42–2.15 ng/mL and 1.41–6.50 ng/mL, respectively. Meanwhile, the RSDs of intra-day and inter-day precision were in the range of 1.43%–5.55% and 4.56%–7.01%, respectively. The spiked recoveries of four purines in real sample were 70.01%–102.42%, indicating this proposed method might have potential applications for the analysis of purines in real samples. In addition, the developed method was used to monitor the concentrations of adenine in rat plasma at different time points after intragastric administration.

KeywordMagnetic Solid Phase Extraction Purines Cupric Ion Polydopamine Plasma
DOI10.1016/j.jchromb.2019.04.054
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000470042300012
PublisherELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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Document TypeJournal article
专题Institute of Chinese Medical Sciences
Corresponding AuthorFeng-Qing Yang; Yi-Tao Wang
Affiliation1.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
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Dong-Dong Zhou,Qian Zhang,Hao Zhang,et al. Cupric ion functionalized polydopamine coated magnetic microspheres as solid-phase adsorbent for the extraction of purines in plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1120:95-103.
APA Dong-Dong Zhou.,Qian Zhang.,Hao Zhang.,Yin-Zhen Wang.,Feng-Qing Yang.,...&Yi-Tao Wang.(2019).Cupric ion functionalized polydopamine coated magnetic microspheres as solid-phase adsorbent for the extraction of purines in plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1120,95-103.
MLA Dong-Dong Zhou,et al."Cupric ion functionalized polydopamine coated magnetic microspheres as solid-phase adsorbent for the extraction of purines in plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1120(2019):95-103.
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