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Platycodin D triggers the extracellular release of programed death Ligand-1 in lung cancer cells
Mu-Yang Huang1; Xiao-Ming Jiang1; Yu-Lian Xu1; Luo-Wei Yuan1; Yu-Chi Chen1; Guozhen Cui2; Run-Yue Huang3; Bo Liu3; Yitao Wang1; Xiuping Chen1; Jin-Jian Lu1
2019-05-28
Source PublicationFOOD AND CHEMICAL TOXICOLOGY
ISSN0278-6915
Volume131
Abstract

Programmed death ligand-1 (PD-L1) is an important immune checkpoint for cancer immunotherapy in clinic. In this study, we reported that platycodin D, a natural product isolated from an edible and medicinal plant Platycodon grandiflorus (Jacq.) A. DC., down-regulated the protein level of PD-L1 in lung cancer cells. Flow cytometry and immunofluorescence assay showed a weaker surface PD-L1 signal in NCI–H1975 cells after the incubation with platycodin D (10 μM) for 15 min compared to the control group. Jurkat T cells showed enhancive interleukin-2 secretion when co-cultured with platycodin D-treated NCI–H1975 cells, suggesting that platycodin D-induced PD-L1 reduction increases the activation of Jurkat T cells. An augmentation of PD-L1 protein was detected in the cell culture medium from platycodin D treatment group. Chlorpromazine (60 μM) almost abolished the platycodin D-mediated PD-L1 extracellular release and restored the membrane PD-L1. Finally, hemolysis assay exhibited that platycodin D-triggered PD-L1 extracellular release was independent of the hemolytic mechanism. Taken together, our study demonstrates that platycodin D reduces the protein level of PD-L1 in lung cancer cells via triggering its release into the cell culture medium, which sheds new light for the application of natural products in cancer immunotherapy.

KeywordProgrammed Death Ligand-1 Platycodin d Extracellular Release Natural Products Cancer
DOI10.1016/j.fct.2019.05.045
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology ; Toxicology
WOS SubjectFood Science & Technology ; Toxicology
WOS IDWOS:000482246600042
PublisherPERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorXiao-Ming Jiang; Jin-Jian Lu
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai, China
3.The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Mu-Yang Huang,Xiao-Ming Jiang,Yu-Lian Xu,et al. Platycodin D triggers the extracellular release of programed death Ligand-1 in lung cancer cells[J]. FOOD AND CHEMICAL TOXICOLOGY,2019,131.
APA Mu-Yang Huang.,Xiao-Ming Jiang.,Yu-Lian Xu.,Luo-Wei Yuan.,Yu-Chi Chen.,...&Jin-Jian Lu.(2019).Platycodin D triggers the extracellular release of programed death Ligand-1 in lung cancer cells.FOOD AND CHEMICAL TOXICOLOGY,131.
MLA Mu-Yang Huang,et al."Platycodin D triggers the extracellular release of programed death Ligand-1 in lung cancer cells".FOOD AND CHEMICAL TOXICOLOGY 131(2019).
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