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Comprehensively qualitative and quantitative analysis of ginsenosides in Panax notoginseng leaves by online two-dimensional liquid chromatography coupled to hybrid linear ion trap Orbitrap mass spectrometry with deeply optimized dilution and modulation system
Ji-Liang Cao1,2,3; Li-Juan Ma2; Sheng-Peng Wang2; Yun Deng3; Yi-Tao Wang2; Peng Li2; Jian-Bo Wan2,3
2019-06-19
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume1079Pages:237-251
Abstract

Panax notoginseng leaves (PNL) was considered as a potential medicinal part with abundant protopanaxdiol type ginsenosides. In this study, an integrated system was developed for simultaneously qualitative and quantitative analysis of ginsenosides in PNL using online comprehensive twodimensional hydrophilic interaction chromatography and reversed-phase liquid chromatography coupled to a hybrid linear ion trap-Orbitrap mass spectrometry (online HILIC RP-ESI/HRMS/MSn). The system was configured based on the combination of a XBridge amide column (150 mm 2.1 mm, 2.5 mm) and Accucore phenyl-hexyl (50 mm 4.6 mm, 2.6 mm) for the first and second dimensions, respectively. An additional water phase was introduced to dilute the eluent from the first dimension to decrease its elution strength in the second dimension. The online dilution, modulation interface and the seconddimension gradient program were deeply optimized to reduce possible sample loss and improve system resolution. Under the optimal conditions, a total of 226 ginsenosides were unambiguously identified or tentatively characterized by aid of high-resolution accurate mass and MSn fragment data in both negative and positive ion modes, and 93 of them were discovered as potentially new ginsenosides in PNL. Besides, the validated online HILIC RP-LTQ-MS method was applied to determine 24 ginsenosides directly on 2D-EIC contour plots in nine batches of PNL samples. The powerful separation capability acquired by the developed online HILIC RP system affords not only reliable structural information for identification, but also accurate quantitation. This combined system can also be used to characterize and quantify bioactive ingredients in the samples with complex matrices.

KeywordOnline Dilution Hilic×rp Hybrid Ltq-orbitrap Mass Spectrometry Chemical Profiling And Quantitation Panax Notoginseng Leaves Ginsenosides
DOI10.1016/j.aca.2019.06.040
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000478681100025
PublisherELSEVIER, RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS
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Document TypeJournal article
专题Institute of Chinese Medical Sciences
Corresponding AuthorPeng Li; Jian-Bo Wan
Affiliation1.PU-UM Innovative Institute of Chinese Medical Sciences, Guangdong-Macau Traditional Chinese Medicine Technology Industrial Park Development Co., Ltd, Hengqin New Area, Zhuhai, Guangdong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China
3.College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
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GB/T 7714
Ji-Liang Cao,Li-Juan Ma,Sheng-Peng Wang,et al. Comprehensively qualitative and quantitative analysis of ginsenosides in Panax notoginseng leaves by online two-dimensional liquid chromatography coupled to hybrid linear ion trap Orbitrap mass spectrometry with deeply optimized dilution and modulation system[J]. ANALYTICA CHIMICA ACTA,2019,1079:237-251.
APA Ji-Liang Cao.,Li-Juan Ma.,Sheng-Peng Wang.,Yun Deng.,Yi-Tao Wang.,...&Jian-Bo Wan.(2019).Comprehensively qualitative and quantitative analysis of ginsenosides in Panax notoginseng leaves by online two-dimensional liquid chromatography coupled to hybrid linear ion trap Orbitrap mass spectrometry with deeply optimized dilution and modulation system.ANALYTICA CHIMICA ACTA,1079,237-251.
MLA Ji-Liang Cao,et al."Comprehensively qualitative and quantitative analysis of ginsenosides in Panax notoginseng leaves by online two-dimensional liquid chromatography coupled to hybrid linear ion trap Orbitrap mass spectrometry with deeply optimized dilution and modulation system".ANALYTICA CHIMICA ACTA 1079(2019):237-251.
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