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Comparison of antioxidant activities of different parts from snow chrysanthemum (Coreopsis tinctoria Nutt.) and identification of their natural antioxidants using high performance liquid chromatography coupled with diode array detection and mass spectrometry and 2,2 '-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt-based assay
L. X. Chen1; D. J. Hu1; S. C. Lam1; L. Ge1; D. Wu2; J. Zhao1; Z. R. Long3; W. J. Yang3; B. Fan4; S. P. Li1
2015-10-19
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1428Pages:134-142
Abstract

Snow chrysanthemum (Coreopsis tinctoria Nutt.), a world-widely well-known flower tea material,has attracted more and more attention because of its beneficial health effects such as antioxidant activity and special flavor. In this study, a high performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) and 2,2'-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt (ABTS) based assay was employed for comparison and identification of antioxidants in different samples of snow chrysanthemum. The results showed that snow chrysanthemum flowers possessed the highest while stems presented the lowest antioxidant capacities. Fourteen detected peaks with antioxidant activity were temporarily identified as 3,4',5,6,7-pentahydroxyflavanone-O-hexoside, chlorogenic acid, 2R-3',4',8-trihydroxyflavanone-7-O-glucoside, flavanomarein, flavanocorepsin, flavanokanin, quercetagitin-7-O-glucoside, 3',5,5',7-tetrahydroxyflavanone-O-hexoside, marein, maritimein, 1,3-dicaffeoylquinic acid, coreopsin, okanin and acetyl-marein by comparing their UV spectra, retention times and MS data with standards or literature data. Antioxidants existed in snow chrysanthemum are quite different from those reported in Chrysanthemum morifolium, a well-known traditional beverage in China, which indicated that snow chrysanthemum may be a promising herbal tea material with obvious antioxidant activity. (C) 2015 Elsevier B.V. All rights reserved.

KeywordCoreopsis Tinctoria Antioxidant Activity Hplc Online Abts-based Assay
DOIhttp://dx.doi.org/10.1016/j.chroma.2015.10.037
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
CSCD IDWOS:000368564000011
PublisherELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorJ. Zhao; S. P. Li
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
2.China Science and Technology Exchange Center, Beijing 100045, China
3.Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute, Urumqi 830011, China
4.Urumqi Jiangqi Agriculture Development Co. Ltd., Urumqi 830011, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
L. X. Chen,D. J. Hu,S. C. Lam,et al. Comparison of antioxidant activities of different parts from snow chrysanthemum (Coreopsis tinctoria Nutt.) and identification of their natural antioxidants using high performance liquid chromatography coupled with diode array detection and mass spectrometry and 2,2 '-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt-based assay[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1428:134-142.
APA L. X. Chen.,D. J. Hu.,S. C. Lam.,L. Ge.,D. Wu.,...&S. P. Li.(2015).Comparison of antioxidant activities of different parts from snow chrysanthemum (Coreopsis tinctoria Nutt.) and identification of their natural antioxidants using high performance liquid chromatography coupled with diode array detection and mass spectrometry and 2,2 '-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt-based assay.JOURNAL OF CHROMATOGRAPHY A,1428,134-142.
MLA L. X. Chen,et al."Comparison of antioxidant activities of different parts from snow chrysanthemum (Coreopsis tinctoria Nutt.) and identification of their natural antioxidants using high performance liquid chromatography coupled with diode array detection and mass spectrometry and 2,2 '-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt-based assay".JOURNAL OF CHROMATOGRAPHY A 1428(2015):134-142.
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