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High-Performance Thin-Layer Chromatographic Fingerprints of Triterpenoids for Distinguishing Between Isodon lophanthoides and Isodon lophanthoides var. gerardianus
Chaozhan Lin1; Fangle Liu1; Runjing Zhang1; Meiting Liu2; Chenchen Zhu 11; Jing Zhao2; Shaoping Li2
2019-06
Source PublicationJOURNAL OF AOAC INTERNATIONAL
ISSN1060-3271
Volume102Issue:3Pages:714-719
Abstract

Background: The aerial parts of lsodon lophanthoides (Buch. Ham. ex D. Don) Hara (IL) has been officially recorded as lsodonis lophanthoidis herba by many provincial quality control standards for Chinese herbal medicines in China. Recently, it has been found that one of its varieties, I. Iophanthoides var. gerardianus (Benth.) Hara (ILVG) was pretended to be I. lophanthoidis herba in herbal material markets or cultivated bases. Because of the similarity on appearance, these two-close related species were difficult to be identified by morphological characters, especially when they are dried and sliced. Objective: To establish a rapid and specific method for identification of the two herbal medicines. Method: In this paper, a fingerprint of triterpenoids by HPTLC coupled with a digital profiling was established to identify IL and distinguish it from its substitute, ILVG. The specific HPTLC fingerprints constructed by determining twelve batches of IL samples and thirteen batches of ILVG samples, intuitionally reflected the difference between the two species on HPTLC image and the peak-peak rations of chemical distribution. Results: Authentication results of nine batches of commercial samples by the above established HPTLC fingerprints exhibited coincident conclusion with that by morphological means. Conclusions: The HPTLC fingerprint is proven to be simple, repeatable, specific, and suitable for rapid identification of I. lophanthoidis herba. Highlights: An efficient method for identification and distinguishing lsodon lophanthoides from its substitute, I. lophanthoides var. gerardianus, was established. HPTLC fingerprints of ursane-type triterpenoides were constructed and validated by determining IL and ILVG samples.

KeywordLiquid-chromatography Quality Assessment Radix
DOI10.5740/jaoacint.18-0305
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Food Science & Technology
WOS SubjectChemistry, Analytical ; Food Science & Technology
WOS IDWOS:000467667900006
PublisherAOAC INT, 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA
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Document TypeJournal article
专题Institute of Chinese Medical Sciences
Corresponding AuthorChenchen Zhu 1
Affiliation1.Guangzhou University of Chinese Medicine, Institute of Clinical Pharmacology, Baiyun Rd, Guangzhou, China 510405
2.University of Macau, State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, N22-Research Building University Rd, Macau, China
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Chaozhan Lin,Fangle Liu,Runjing Zhang,et al. High-Performance Thin-Layer Chromatographic Fingerprints of Triterpenoids for Distinguishing Between Isodon lophanthoides and Isodon lophanthoides var. gerardianus[J]. JOURNAL OF AOAC INTERNATIONAL,2019,102(3):714-719.
APA Chaozhan Lin.,Fangle Liu.,Runjing Zhang.,Meiting Liu.,Chenchen Zhu 1.,...&Shaoping Li.(2019).High-Performance Thin-Layer Chromatographic Fingerprints of Triterpenoids for Distinguishing Between Isodon lophanthoides and Isodon lophanthoides var. gerardianus.JOURNAL OF AOAC INTERNATIONAL,102(3),714-719.
MLA Chaozhan Lin,et al."High-Performance Thin-Layer Chromatographic Fingerprints of Triterpenoids for Distinguishing Between Isodon lophanthoides and Isodon lophanthoides var. gerardianus".JOURNAL OF AOAC INTERNATIONAL 102.3(2019):714-719.
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