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Studying the time course of cardiac responses of the same zebrafish using scalable fish-dock microarchitecture
Guodong Yu; Simon Ming-Yuen Lee; Cheuk-Wing Li
2018-09
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume268Pages:245-254
Abstract

In conventional zebrafish (Danio rerio) studies, fishes are sacrificed at individual time points, so it is impossible to analyze the time-course responses of the same zebrafish. To tackle this problem, a PMMA-based microfluidic "Fish-dock" featuring scalable embryo loading, motility-based selection, orientation control, and drug dispensing functionalities was devised. Single zebrafish embryos were isolated and high-quality larvae were selected through an on-chip transfer mechanism for in vivo drug screening. Dorsal orientation of the larvae was gently and reversibly maintained by the "Fish-dock" to reduce motion artifacts during heartbeat recording. By repeatedly docking the same zebrafish at different time points, this microchip enabled parallel tracking of the cardiac function among 48 individual larvae for time course experiments lasting over 48 h. Compared with conventional analysis method, the time-course normalized single-larva analyses revealed the similar dose dependence of diphenylurea (DPU) in treating doxorubicin (Dox)-induced cardiomyopathy, yet the drug treatment time was reduced by half compared with that conventionally required to reveal such a trend.

KeywordSingle-fish Analysis Water-level Docking Time-course Normalization Diphenylurea Doxorubicin-induced Cardiomyopathy
DOI10.1016/j.snb.2018.04.084
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000432776800033
PublisherELSEVIER SCIENCE SA
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorCheuk-Wing Li
AffiliationInstitute of Chinese Medical Sciences, University of Macau, Macau SAR, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Guodong Yu,Simon Ming-Yuen Lee,Cheuk-Wing Li. Studying the time course of cardiac responses of the same zebrafish using scalable fish-dock microarchitecture[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,268:245-254.
APA Guodong Yu,Simon Ming-Yuen Lee,&Cheuk-Wing Li.(2018).Studying the time course of cardiac responses of the same zebrafish using scalable fish-dock microarchitecture.SENSORS AND ACTUATORS B-CHEMICAL,268,245-254.
MLA Guodong Yu,et al."Studying the time course of cardiac responses of the same zebrafish using scalable fish-dock microarchitecture".SENSORS AND ACTUATORS B-CHEMICAL 268(2018):245-254.
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