UM  > 中華醫藥研究院
Baicalein Induces Beclin 1-and Extracellular Signal-Regulated Kinase-Dependent Autophagy in Ovarian Cancer Cells
Wang, Ya-Fang1; Xu, Yu-Lian1; Tang, Zheng-Hai1; Li, Ting1; Zhang, Le-Le1; Chen, Xiuping1; Lu, Jia-Hong1; Leung, Chung-Hang1; Ma, Dik-Lung1; Qiang, Wen-An2; Wang, Yi-Tao1; Lu, Jin-Jian1
2017
Source PublicationAMERICAN JOURNAL OF CHINESE MEDICINE
ISSN0192-415X
Volume45Issue:1Pages:123-136
Abstract

Baicalein (BA), one of the major compounds isolated from the root of Scutellaria baicalensis Gerogi, exhibits various pharmacological effects, such as anti-oxidant, anti-inflammatory, and anticancer effects. In this study, we found that BA reduced cell viability and increased apoptosis in ovarian cancer cells. Treatment of cells with BA enhanced microtubule-associated protein light chain 3-II (LC3-II) expression, acidic vesicular organelle and GFP-LC3 fluorescence dot accumulation. Combined treatment with chloroquine and BA apparently reduced cell viability and increased the cleavage of poly (ADPribose) polymerase (PARP) in both HEY and A2780 ovarian cancer cell lines, indicating that BA induces a protective autophagy in these cells. Knockdown of Beclin 1 by siRNA remarkably decreased BA-induced LC3-II lipidation. In addition, we found an increase in the phosphorylation of extracellular signal-regulated kinase (ERK, Thr202/ Thr204) and AKT (Ser473) after BA treatment, and inhibition of ERK activation by the pharmacological inhibitor U0126 or ERK siRNA blocked BA-induced autophagy. Taken together, these results suggest that BA induces Beclin 1- and ERK-dependent autophagy in ovarian cancer cells.

KeywordBaicalein Ovarian Cancer Autophagy Beclin 1 Erk
DOI10.1142/S0192415X17500094
URLView the original
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine ; General & Internal Medicine
WOS SubjectIntegrative & Complementary Medicine ; Medicine, General & Internal
WOS IDWOS:000395376800009
PublisherWORLD SCIENTIFIC PUBL CO PTE LTD
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, P.R. China
2.Department of Obstetrics and Gynecology-Division of Reproductive Science in Medicine, Department of Pathology, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wang, Ya-Fang,Xu, Yu-Lian,Tang, Zheng-Hai,et al. Baicalein Induces Beclin 1-and Extracellular Signal-Regulated Kinase-Dependent Autophagy in Ovarian Cancer Cells[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2017,45(1):123-136.
APA Wang, Ya-Fang.,Xu, Yu-Lian.,Tang, Zheng-Hai.,Li, Ting.,Zhang, Le-Le.,...&Lu, Jin-Jian.(2017).Baicalein Induces Beclin 1-and Extracellular Signal-Regulated Kinase-Dependent Autophagy in Ovarian Cancer Cells.AMERICAN JOURNAL OF CHINESE MEDICINE,45(1),123-136.
MLA Wang, Ya-Fang,et al."Baicalein Induces Beclin 1-and Extracellular Signal-Regulated Kinase-Dependent Autophagy in Ovarian Cancer Cells".AMERICAN JOURNAL OF CHINESE MEDICINE 45.1(2017):123-136.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Ya-Fang]'s Articles
[Xu, Yu-Lian]'s Articles
[Tang, Zheng-Hai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Ya-Fang]'s Articles
[Xu, Yu-Lian]'s Articles
[Tang, Zheng-Hai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Ya-Fang]'s Articles
[Xu, Yu-Lian]'s Articles
[Tang, Zheng-Hai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.