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Comprehensive Quantitative Analysis of 32 Chemical Ingredients of a Chinese Patented Drug Sanhuang Tablet
Fung, Hau-Yee1; Lang, Yan2; Ho, Hing-Man1; Wong, Tin-Long1; Ma, Dik-Lung3; Leung, Chung-Hang4; Han, Quan-Bin1
2017-01
Source PublicationMOLECULES
ISSN1420-3049
Volume22Issue:1
Abstract

Sanhuang Tablet (SHT) is a Chinese patented drug commonly used for the treatment of inflammations of the respiratory tract, gastrointestinal tract, and skin. It contains a special medicinal composition including the single compound berberine hydrochloride, extracts of Scutellariae Radix and Rhei Radix et Rhizoma, as well as the powder of Rhei Radix et Rhizoma. Despite advances in analytical techniques, quantitative evaluation of a Chinese patented drug like SHT remains a challenge due to the complexity of its chemical profile. In this study, ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was used to simultaneously quantify 29 non-sugar small molecule components of SHT (11 flavonoids, two isoflavonoids, one flavanone, five anthraquinones, two dianthranones, five alkaloids, two organic acids and one stilbene). Three major saccharide components, namely fructose, glucose, and sucrose, were also quantitatively determined using high performance liquid chromatography-charged aerosol detector (HPLC-CAD) on an Asahipak NH2P-50 4E amino column. The established methods were validated in terms of linearity, sensitivity, precision, accuracy, and stability, and then successfully applied to analyze 27 batches of commercial SHT products. A total of up to 57.61% (w/w) of SHT could be quantified, in which the contents of the determined non-saccharide small molecules varied from 5.91% to 16.83% (w/w) and three saccharides accounted for 4.41% to 48.05% (w/w). The results showed that the quality of the commercial products was inconsistent, and only four of those met Chinese Pharmacopoeia criteria.

KeywordSanhuang Tablet Chinese Patented Drug Quantitative Analysis Quality Evaluation Cad
DOI10.3390/molecules22010111
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000395473500111
PublisherMDPI AG
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Co-First AuthorFung, Hau-Yee
Affiliation1.School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
2.First Affiliated Hospital, Zhengzhou University, Zhengzhou 450001, China
3.Department of Chemistry, Hong Kong Baptist University, Hong Kong, China
4.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Recommended Citation
GB/T 7714
Fung, Hau-Yee,Lang, Yan,Ho, Hing-Man,et al. Comprehensive Quantitative Analysis of 32 Chemical Ingredients of a Chinese Patented Drug Sanhuang Tablet[J]. MOLECULES,2017,22(1).
APA Fung, Hau-Yee.,Lang, Yan.,Ho, Hing-Man.,Wong, Tin-Long.,Ma, Dik-Lung.,...&Han, Quan-Bin.(2017).Comprehensive Quantitative Analysis of 32 Chemical Ingredients of a Chinese Patented Drug Sanhuang Tablet.MOLECULES,22(1).
MLA Fung, Hau-Yee,et al."Comprehensive Quantitative Analysis of 32 Chemical Ingredients of a Chinese Patented Drug Sanhuang Tablet".MOLECULES 22.1(2017).
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