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Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium
Chen, Guokai1,2; Rao, Mahendra3,4,5
2017
Source PublicationSTEM CELL BANKING: CONCEPTS AND PROTOCOLS
ISSN1064-3745
Volume1590Pages:131-137
Abstract

Human somatic cells can be reprogrammed by defined factors to induced pluripotent stem cells (iPSCs). Importantly, the quality of iPSCs could impact the potential of these cells in basic and clinic research. Here, we describe a method to reprogram human fibroblast cells with Sendai virus in chemically defined conditions, to generate iPSCs that are integration-free and suitable for research and translational applications.

KeywordInduced Pluripotent Stem Cells Reprogramming Chemically Defined Sendai Virus
DOIhttp://doi.org/10.1007/978-1-4939-6921-0_9
URLView the original
Indexed ByBSCI ; BSCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000417820100010
PublisherHUMANA PRESS INC
The Source to ArticleWOS
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorChen, Guokai; Rao, Mahendra
Affiliation1.Faculty of Health SciencesUniversity of MacauTaipa, MacauChina
2.Center for Molecular Medicine, National HeartLung and Blood InstituteBethesdaUSA
3.New York Stem Cell Foundation Research Institute,New York,USA
4.Q Therapeutics,Salt Lake City USA
5.Wake Forest Institute for Regenerative MedicineWake Forest UniversityWinston-SalemUSA
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Chen, Guokai,Rao, Mahendra. Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium[J]. STEM CELL BANKING: CONCEPTS AND PROTOCOLS,2017,1590:131-137.
APA Chen, Guokai,&Rao, Mahendra.(2017).Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium.STEM CELL BANKING: CONCEPTS AND PROTOCOLS,1590,131-137.
MLA Chen, Guokai,et al."Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium".STEM CELL BANKING: CONCEPTS AND PROTOCOLS 1590(2017):131-137.
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