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Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis
Fu, Wen-Juan1; Lei, Ting2; Yin, Zhen2; Pan, Jian-Hao1; Chai, Yu-Shuang2; Xu, Xiao-Yun1; Yan, Yi-Xi1; Wang, Zhi-Hua1; Ke, Jian1; Wu, Gang2; Xu, Ren-He3; Paranjpe, Manish4; Qu, Lintao5; Nie, Hong1

Ethnopharmacological relevance: The Angong Niuhuang Pill (ANP) is a well known Chinese traditional therapeutic for the treatment for diseases affecting the Central Nervous System (CNS). Components of the ANP formulation, including Bovis Calculus Sativus, Pulvis Bubali Comus Concentratus, Moschus, Margarita, Cinnabaris, Realgar, Coptidis Rhizoma, Scutellariae Radix, Gardeniae Fructus, Curcumae Radix, and Bomeolum Syntheticum, have been used for the treatment of stroke, encephalitis and emergency meningitis across Asia, especially in China for hundreds of years. Objective: The goal of this study was to investigate the anti-atherosclerosis and cardio-protective effects of ANP administration using a rodent model of atherosclerosis induced by a high fat and vitamin D3. Methods: Specific Pathogen-Free (SPF) 78 male SD rats were randomly divided into a control group and 5 atherosclerotic model groups. The atherosclerotic groups were divided to receive either Simvastatin (SVTT, 0.005 g/kg), Low-dose ANP (0.125 g/kg), Medium-dose ANP (0.25 g/kg), and High-dose ANP (0.5 g/kg). Following adaptive feeding for one week, atherosclerosis was induced and the atherosclerosis model was established. Experimental drugs (either simvastatin or ANP) or normal saline were administered intragastrically once daily for 9 weeks starting from the 8th week. A carotid artery ultrasound was performed at the 17th week to determine whether atherosclerosis had been induced. After the atherosclerosis model was successfully established, platelet aggregation rates, serum biochemical indices, apoptosis-related Bcl-2, Bax proteins levels in the heart were assayed. Pathological and histological analysis was completed using artery tissue from different experimental different groups to assess the effects of ANP. Results: ANP significantly decreased aortic membrane thickness, the maximum platelet aggregation rates, and the ratio of low density lipoprotein cholesterol (LDL) to high density lipoprotein cholesterol (HDL). In addition, ANP significantly reduced serum contents of total cholesterol, low density lipoprotein, malondialdehyde, troponin I, high-sensitivity C-reactive protein, and lactate dehydrogenase. ANP markedly improved abnormal pathological conditions of the aorta and heart, and helped to prevent myocardial apoptosis. Conclusions: We have demonstrated that ANP has robust ant-atherosclerosis and cardio-protective effects on a high-fat and vitamin D3 – induced rodent model of atherosclerosis due to its antiplatelet aggregation, lipid regulatory, antioxidant, anti-inflammatory and anti-apoptotic properties.

KeywordAngong Niuhuang Pill (Anp) Atherosclerosis High Fat Diet Vitamin D-3
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Indexed BySCI
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000392897300010
The Source to ArticleWOS
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Cited Times [WOS]:18   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Co-First AuthorFu, Wen-Juan
Corresponding AuthorNie, Hong
Affiliation1.Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, Guangdong, China
2.Guangzhou Baiyunshan Zhongyi pharmaceutical co., ltd, Guangzhou 510530, Guangdong, China
3.Health Sciences, University of Macau, Taipa, 999000 Macau, China
4.Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA
5.Department of Neurosurgery, Neurosurgery pain research institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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GB/T 7714
Fu, Wen-Juan,Lei, Ting,Yin, Zhen,et al. Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis[J]. JOURNAL OF ETHNOPHARMACOLOGY,2017,195:118-126.
APA Fu, Wen-Juan.,Lei, Ting.,Yin, Zhen.,Pan, Jian-Hao.,Chai, Yu-Shuang.,...&Nie, Hong.(2017).Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis.JOURNAL OF ETHNOPHARMACOLOGY,195,118-126.
MLA Fu, Wen-Juan,et al."Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis".JOURNAL OF ETHNOPHARMACOLOGY 195(2017):118-126.
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