UM
Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes
Lin, Yongshun; Linask, Kaari L.; Mallon, Barbara; Johnson, Kory; Klein, Michael; Beers, Jeanette; Xie, Wen; Du, Yubin; Liu, Chengyu; Lai, Yinzhi; Zou, Jizhong; Haigney, Mark; Yang, Hushan; Rao, Mahendra; Chen, Guokai
2017-02
Source PublicationSTEM CELLS TRANSLATIONAL MEDICINE
ISSN2157-6564
Volume6Issue:2Pages:527-538
AbstractCardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium, we identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and developed an efficient method to differentiate hPSCs into cardiomyocytes. The treatment with heparin helped cardiomyocyte differentiation consistently reach at least 80% purity (up to 95%) from more than 10 different hPSC lines in chemically defined Dulbecco's modified Eagle's medium/F-12-based medium on either Matrigel or defined matrices like vitronectin and Synthemax. One of heparin's main functions was to act as a Wnt modulator that helped promote robust and consistent cardiomyocyte production. Our study provides an efficient, reliable, and cost-effective method for cardiomyocyte derivation from hPSCs that can be used for potential large-scale drug screening, disease modeling, and future cellular therapies. (C) STEM CELLS TRANSLATIONAL MEDICINE 2017; 6:527-538
KeywordCardiac Cell culture Embryonic stem cells Induced pluripotent stem cells Heparin
DOI10.5966/sctm.2015-0428
URLView the original
Indexed BySCI
Language英语
WOS Research AreaCell Biology
WOS SubjectCell & Tissue Engineering
WOS IDWOS:000398198500018
PublisherWILEY
The Source to ArticleWOS
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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GB/T 7714
Lin, Yongshun,Linask, Kaari L.,Mallon, Barbara,et al. Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes[J]. STEM CELLS TRANSLATIONAL MEDICINE,2017,6(2):527-538.
APA Lin, Yongshun.,Linask, Kaari L..,Mallon, Barbara.,Johnson, Kory.,Klein, Michael.,...&Chen, Guokai.(2017).Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes.STEM CELLS TRANSLATIONAL MEDICINE,6(2),527-538.
MLA Lin, Yongshun,et al."Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes".STEM CELLS TRANSLATIONAL MEDICINE 6.2(2017):527-538.
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