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A cyclometalated iridium(III) complex used as a conductor for the electrochemical sensing of IFN-gamma
Miao, Xiangmin1,2; Ko, Chung-Nga2; Vellaisamy, Kasipandi2; Li, Zongbing1; Yang, Guanjun3; Leung, Chung-Hang3; Ma, Dik-Lung2
2017-02-15
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7
Abstract

A novel iridium(III) complex was prepared and used as a conductor for sensitive and enzyme-free electrochemical detection of interferon gamma (IFN-gamma). This assay is based on a dual signal amplification mechanism involving positively charged gold nanoparticles ((+) AuNPs) and hybridization chain reaction (HCR). To construct the sensor, nafion (Nf) and (+) AuNPs composite membrane was first immobilized onto the electrode surface. Subsequently, a loop-stem structured capture probe (CP) containing a special IFN-gamma interact strand was modified onto the (+) AuNP surface via the formation of Au-S bonds. Upon addition of IFN-gamma, the loop-stem structure of CP was opened, and the newly exposed "sticky" region of CP then hybridized with DNA hairpin-1 (H-1), which in turn opened its hairpin structure for hybridizing with DNA hairpin-2 (H-2). Happen of HCR between H-1 and H-2 thus generated a polymeric duplex DNA (dsDNA) chain. Meanwhile, the iridium(III) complex could interact with the grooves of the dsDNA polymer, producing a strong current signal that was proportional to IFN-gamma concentration. Thus, sensitive detection of IFN-gamma could be realized with a detection limit down to 16.3 fM. Moreover, satisfied results were achieved by using this method for the detection of IFN-gamma in human serum samples.

DOI10.1038/srep42740
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000394297500001
PublisherNATURE PUBLISHING GROUP
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:11   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Jiangsu Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China
2.Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China
3.University of Macauu, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
Recommended Citation
GB/T 7714
Miao, Xiangmin,Ko, Chung-Nga,Vellaisamy, Kasipandi,et al. A cyclometalated iridium(III) complex used as a conductor for the electrochemical sensing of IFN-gamma[J]. SCIENTIFIC REPORTS,2017,7.
APA Miao, Xiangmin.,Ko, Chung-Nga.,Vellaisamy, Kasipandi.,Li, Zongbing.,Yang, Guanjun.,...&Ma, Dik-Lung.(2017).A cyclometalated iridium(III) complex used as a conductor for the electrochemical sensing of IFN-gamma.SCIENTIFIC REPORTS,7.
MLA Miao, Xiangmin,et al."A cyclometalated iridium(III) complex used as a conductor for the electrochemical sensing of IFN-gamma".SCIENTIFIC REPORTS 7(2017).
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