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Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy
Qu, Cheng; Wang, Lin-Yan; Lin, Hang; Shang, Er-Xin; Tang, Yu-Ping; Yue, Shi-Jun; Jin, Yi; Tao, Wei-Wei; Li, Shao-Ping; Hua, Yong-Qing; Liu, Pei; Su, Shu-Lan; Zhou, Huiping; Qian, Da-Wei; Duan, Jin-Ao
2017-02-20
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
Volume135Pages:206-216
AbstractA novel and generally applicable approach was established to hierarchically identify the bioactive components of a medicinal herb by preparative high-performance liquid chromatography (prep-HPLC) and a selective knock-out strategy. In this study, the targeted components of an herbal medicine were separated and knocked out using prep-HPLC. Subsequently, the contributions of the different target components to the overall effect of the medicinal herb were comparatively evaluated and differentiated by a heat map and a 3D score plot. This approach was successfully applied to investigate the bioactive constituents of safflower. The contributions of 11 components to the overall effect of safflower were as follows: anhydrosafflor yellow B(10)>6-hydroxykaempferol 3,6-di-O-beta-D-glucoside (8 > hydroxysafflor yellow A (3)> kaempferol 3-O-beta-rutinoside (11)> 6-hydroxykaempferol 3-O-beta-rutinoside (9)> 6-hydroxykaempferol 3,6-di-O-beta-D-glucoside-7-O-beta-D-glucuronide (4)> 6-hydroxyapigenin 6-O-D-glucoside-7-O-beta-D-glucuronide (6) > cytidine (1)> 6-hydroxykaempferol 3-O-beta-rutinoside-6-O-beta-D-glucoside (7)> 6-hydroxykaempferol 3,6,7-tri-O-beta-D-glucoside (5) > adenosine (2). These results demonstrate that quinochalcone C-glycosides (3 and 10) and some flavonoid glycosides containing C7-OH (such as 8, 9 and 11) made a greater contribution to the overall effect of safflower than the other components that were knocked out. The results provided an important reference for improving quality control and further development of safflower products. And this approach should also be useful for investigating the bioactive constituents of other medicinal herbs. (C) 2016 Elsevier B.V. All rights reserved.
KeywordSafflower Herb Contribution Component High-performance liquid chromatography Knock-out
DOI10.1016/j.jpba.2016.12.027
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000392905200023
PublisherELSEVIER SCIENCE BV
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Qu, Cheng,Wang, Lin-Yan,Lin, Hang,et al. Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,135:206-216.
APA Qu, Cheng.,Wang, Lin-Yan.,Lin, Hang.,Shang, Er-Xin.,Tang, Yu-Ping.,...&Duan, Jin-Ao.(2017).Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,135,206-216.
MLA Qu, Cheng,et al."Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 135(2017):206-216.
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