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Evaluation of interactions between RAW264.7 macrophages and small molecules by capillary electrophoresis
Wang, Feng-Qin1; Li, Qiao-Qiao1; Zhang, Qian1; Wang, Yin-Zhen1; Hu, Yuan-Jia2; Li, Peng2; Wan, Jian-Bo2; Yang, Feng-Qing1; Xia, Zhi-Ning1
2017-03
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume38Issue:6Pages:938-941
Abstract

In this study, the affinity interactions between RAW 264.7 macrophages and three small molecules including naringin, oleuropein and paeoniflorin were evaluated by affinity capillary electrophoresis (ACE), partial filling affinity capillary electrophoresis (PFACE) and frontal analysis capillary electrophoresis (FACE), respectively. The result indicated that ACE (varying concentrations of cell suspension were filled in the capillary as receptor) may not be suitable for the evaluation of interactions between cell and small molecules due to the high viscosity of cell suspension; PFACE can qualitatively evaluate the interaction, but the difference in viscosity between RAW264.7 suspension and buffer effects on the liner relationship between filling length and injection time, which makes the calculation of binding constant difficult. Furthermore, based on the PFACE results, naringin showed stronger interaction with macrophages than the other two molecules; taking advantage of the aggregation phenomenon of cell induced by electric field, FACE was successfully used to determine the stoichiometry (n = 5x10(9)) and binding constant (K-b = 1x10(4) L/mol) of the interaction between RAW264.7 and naringin.

KeywordAffinity Interaction Cell Aggregation Frontal Analysis Capillary Electrophoresis Raw264.7 Macrophage
DOI10.1002/elps.201600345
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000397180300015
PublisherWILEY
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYang, Feng-Qing
Affiliation1.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R., China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, P. R., China
Recommended Citation
GB/T 7714
Wang, Feng-Qin,Li, Qiao-Qiao,Zhang, Qian,et al. Evaluation of interactions between RAW264.7 macrophages and small molecules by capillary electrophoresis[J]. ELECTROPHORESIS,2017,38(6):938-941.
APA Wang, Feng-Qin.,Li, Qiao-Qiao.,Zhang, Qian.,Wang, Yin-Zhen.,Hu, Yuan-Jia.,...&Xia, Zhi-Ning.(2017).Evaluation of interactions between RAW264.7 macrophages and small molecules by capillary electrophoresis.ELECTROPHORESIS,38(6),938-941.
MLA Wang, Feng-Qin,et al."Evaluation of interactions between RAW264.7 macrophages and small molecules by capillary electrophoresis".ELECTROPHORESIS 38.6(2017):938-941.
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