UM  > 健康科學學院
Hemodynamic shear stress stimulates migration and extravasation of tumor cells by elevating cellular oxidative level
Ma, Shijun1; Fu, Afu1; Chiew, Geraldine Giap Ying1; Luo, Kathy Qian2
2017-03
Source PublicationCANCER LETTERS
ISSN0304-3835
Volume388Pages:239-248
Abstract

Cancer cells are shed into the blood stream and are exposed to hemodynamic shear stress during metastasis. It has been shown that shear stress can destroy circulating tumor cells (CTCs) both in vitro and in vivo. However, it remains unclear whether shear stress can modulate the properties and functions of tumor cells in a manner that might help CTCs to exit circulation. In this study, we established a microfluidic circulatory system to apply physiological fluid shear stress on breast cancer cells and demonstrated that an arterial level of shear stress significantly enhanced tumor cell migration in transwell and wound healing assays, and enhanced extravasation in a transendothelial assay. Circulatory treatment elevated the intracellular levels of reactive oxygen species (ROS), which is an early and indispensable event for activating the extracellular signal-regulated kinases (ERK1/2). Subsequently, ERK1/2 activation promoted the migration of tumor cells and enhanced their extravasation. Finally, reducing cellular ROS production suppressed tumor cell extravasation in both a transendothelial assay and a zebrafish model. This new understanding of how fluid shear stress promotes tumor cell migration has important implications in cancer treatment and can help us to identify potential therapeutic targets for inhibiting tumor progression. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

KeywordCirculating Tumor Cells Shear Stress Reactive Oxygen Species Erk1/2 Cell Migration Extravasation
DOI10.1016/j.canlet.2016.12.001
URLView the original
Indexed BySCI
Language英语
WOS Research AreaOncology
WOS SubjectOncology
WOS IDWOS:000395959800024
PublisherELSEVIER IRELAND LTD
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
2.Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
Recommended Citation
GB/T 7714
Ma, Shijun,Fu, Afu,Chiew, Geraldine Giap Ying,et al. Hemodynamic shear stress stimulates migration and extravasation of tumor cells by elevating cellular oxidative level[J]. CANCER LETTERS,2017,388:239-248.
APA Ma, Shijun,Fu, Afu,Chiew, Geraldine Giap Ying,&Luo, Kathy Qian.(2017).Hemodynamic shear stress stimulates migration and extravasation of tumor cells by elevating cellular oxidative level.CANCER LETTERS,388,239-248.
MLA Ma, Shijun,et al."Hemodynamic shear stress stimulates migration and extravasation of tumor cells by elevating cellular oxidative level".CANCER LETTERS 388(2017):239-248.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ma, Shijun]'s Articles
[Fu, Afu]'s Articles
[Chiew, Geraldine Giap Ying]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ma, Shijun]'s Articles
[Fu, Afu]'s Articles
[Chiew, Geraldine Giap Ying]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ma, Shijun]'s Articles
[Fu, Afu]'s Articles
[Chiew, Geraldine Giap Ying]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.