UM  > 健康科學學院
Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy
Huang, Jing-Yu1; Guo, Lun-Zhang1; Wang, Jing-Zun1; Li, Tse-Chung1; Lee, Hsin-Jung2; Chiu, Po-Kai3; Peng, Lung-Han2; Liu, Tzu-Ming1,4,5
2017-03
Source PublicationJOURNAL OF BIOMEDICAL OPTICS
ISSN1083-3668
Volume22Issue:3
Abstract

Harmonic generation microscopy (HGM) has become one unique tool of optical virtual biopsy for the diagnosis of cancer and the in vivo cytometry of leukocytes. Without labeling, HGM can reveal the submicron features of tissues and cells in vivo. For deep imaging depth and minimal invasiveness, people commonly adopt 1100- to 1300-nm femtosecond laser sources. However, those lasers are typically based on bulky oscillators whose performances are sensitive to environmental conditions. We demonstrate a fiber-based 1150-nm femtosecond laser source, with 6.5-nJ pulse energy, 86-fs pulse width, and 11.25-MHz pulse repetition rate. It was obtained by a bismuth borate or magnesium-doped periodically poled lithium niobate (MgO:PPLN) mediated frequency doubling of the 2300-nm solitons, generated from an excitation of 1550-nm femtosecond pulses on a large mode area photonic crystal fiber. Combined with a home-built laser scanned microscope and a tailor-made frame grabber, we achieve a pulse-per-pixel HGM imaging in vivo at a 30-Hz frame rate. This integrated solution has the potential to be developed as a stable HGM system for routine clinical use. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)

KeywordHarmonic Generation Microscopy Soliton Self-frequency-shift Photonic Crystal Fiber
DOIhttp://doi.org/10.1117/1.JBO.22.3.036008
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectBiochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000397944900023
PublisherSPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorLiu, Tzu-Ming
Affiliation1.National Taiwan University, Institute of Biomedical Engineering, Taipei, Taiwan
2.National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Taipei, Taiwan
3.Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu, Taiwan
4.University of Macau, Faculty of Health Sciences, Taipa, Macao SAR, China
5.National Taiwan University, Molecular Imaging Center, Taipei, Taiwan
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Huang, Jing-Yu,Guo, Lun-Zhang,Wang, Jing-Zun,et al. Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy[J]. JOURNAL OF BIOMEDICAL OPTICS,2017,22(3).
APA Huang, Jing-Yu.,Guo, Lun-Zhang.,Wang, Jing-Zun.,Li, Tse-Chung.,Lee, Hsin-Jung.,...&Liu, Tzu-Ming.(2017).Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy.JOURNAL OF BIOMEDICAL OPTICS,22(3).
MLA Huang, Jing-Yu,et al."Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy".JOURNAL OF BIOMEDICAL OPTICS 22.3(2017).
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, Jing-Yu]'s Articles
[Guo, Lun-Zhang]'s Articles
[Wang, Jing-Zun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, Jing-Yu]'s Articles
[Guo, Lun-Zhang]'s Articles
[Wang, Jing-Zun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, Jing-Yu]'s Articles
[Guo, Lun-Zhang]'s Articles
[Wang, Jing-Zun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.