UM
Alisol A 24-Acetate and Alisol B 23-Acetate Induced Autophagy Mediates Apoptosis and Nephrotoxicity in Human Renal Proximal Tubular Cells
Wang, Chunfei; Feng, Liang; Ma, Liang; Chen, Haifeng; Tan, Xiaobin; Hou, Xuefeng; Song, Jie; Cui, Li; Liu, Dan; Chen, Juan; Yang, Nan; Wang, Jing; Liu, Ying; Zhao, Bingjie; Wang, Gang; Zhou, Yuanli; Jia, Xiaobin
2017-03-31
Source PublicationFRONTIERS IN PHARMACOLOGY
ISSN1663-9812
Volume8
AbstractTwo natural compounds alisol A 24-acetate (24A) and alisol B 23-acetate (23B) are abundant in Rhizoma alismatis. In the present study, we evaluated the induction of 24A and 23B on apoptosis and possible nephrotoxicity of human renal proximal tubular (HK-2) cells by activating autophagy and also explored its regulation on PI3K/Akt/mTOR signaling pathway. Presently, Clusterin, Kim-1, and TFF-3 were considered to be new bioindicators of nephrotoxicity. Interestingly, the protein expression and mRNA levels of Clusterin, Kim-1 and TFF-3 could be significantly increased by 23B and 24A in vivo and in vitro. Furthermore, cell apoptosis could be triggered by 23B and 24A via significantly decreasing the protein expression and mRNA levels of Bcl-2 and Bcl-xl. Autophagy of HK-2 cells could be induced by both 23B and 24A via significantly enhancing the ratio of LC3II/LC3I, the protein expression of Beclin-1 as well as the mRNA levels of LC3 and Beclin-1. Meanwhile, PI3K/Akt/mTOR signaling pathway could be inhibited by these two compounds. An autophagy inhibitor, 3-methyladenine, could partially reverse cell viability and conversely change the ratio of LC3II/LC3I and the protein expression of Bcl-2 and Kim-1. Thus this study helped to understand that 23B and 24A induced autophagy resulted in apoptosis and nephrotoxicity through inhibiting PI3K/Akt/mTOR signaling pathway, facilitating further studies for nephrotoxicity induced by these two compounds and could be beneficial for safe use of Rhizoma alismatis in clinic.
Keywordalisol A 24-acetate alisol B 23-acetate autophagy apoptosis nephrotoxicity
DOI10.3389/fphar.2017.00172
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000397976300001
PublisherFRONTIERS MEDIA SA
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Wang, Chunfei,Feng, Liang,Ma, Liang,et al. Alisol A 24-Acetate and Alisol B 23-Acetate Induced Autophagy Mediates Apoptosis and Nephrotoxicity in Human Renal Proximal Tubular Cells[J]. FRONTIERS IN PHARMACOLOGY,2017,8.
APA Wang, Chunfei.,Feng, Liang.,Ma, Liang.,Chen, Haifeng.,Tan, Xiaobin.,...&Jia, Xiaobin.(2017).Alisol A 24-Acetate and Alisol B 23-Acetate Induced Autophagy Mediates Apoptosis and Nephrotoxicity in Human Renal Proximal Tubular Cells.FRONTIERS IN PHARMACOLOGY,8.
MLA Wang, Chunfei,et al."Alisol A 24-Acetate and Alisol B 23-Acetate Induced Autophagy Mediates Apoptosis and Nephrotoxicity in Human Renal Proximal Tubular Cells".FRONTIERS IN PHARMACOLOGY 8(2017).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Chunfei]'s Articles
[Feng, Liang]'s Articles
[Ma, Liang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Chunfei]'s Articles
[Feng, Liang]'s Articles
[Ma, Liang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Chunfei]'s Articles
[Feng, Liang]'s Articles
[Ma, Liang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.