UM
Identification of four cornua by ultra-performance liquid chromatography with time-of-flight mass spectrometry coupled with principal component analysis
Cheng, Xian-Long; Zhang, Qian-Qian; Li, Ming-Hua; Yan, Hua; Yu, Kun-Zi; Zhou, Jian-Li; Li, Shao-Ping; Wei, Feng; Ma, Shuang-Cheng
2017-04
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume40Issue:8Pages:1667-1673
AbstractAn ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry method coupled with principal component analysis was developed and applied to the identification of Cornu Antelopis, Cornu Bubali, Cornu Naemorhedi, and Cornu Bovis. The data obtained from the trypsin-digested samples were subjected to principal component analysis to classify these four cornua. Additionally, marker peptides of the cornua were determined by orthogonal partial least-squares discriminant analysis, and fragmentation tandem mass spectra of these marker peptides were evaluated. The results from this study indicate that the proposed method is reliable, and it has been successfully applied to the identification of variants of cornua commonly used in traditional Chinese medicine.
Keywordcornua liquid chromatography marker peptides mass spectrometry principal component analysis
DOI10.1002/jssc.201600902
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000399783300005
PublisherWILEY-V C H VERLAG GMBH
The Source to ArticleWOS
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Cheng, Xian-Long,Zhang, Qian-Qian,Li, Ming-Hua,et al. Identification of four cornua by ultra-performance liquid chromatography with time-of-flight mass spectrometry coupled with principal component analysis[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(8):1667-1673.
APA Cheng, Xian-Long.,Zhang, Qian-Qian.,Li, Ming-Hua.,Yan, Hua.,Yu, Kun-Zi.,...&Ma, Shuang-Cheng.(2017).Identification of four cornua by ultra-performance liquid chromatography with time-of-flight mass spectrometry coupled with principal component analysis.JOURNAL OF SEPARATION SCIENCE,40(8),1667-1673.
MLA Cheng, Xian-Long,et al."Identification of four cornua by ultra-performance liquid chromatography with time-of-flight mass spectrometry coupled with principal component analysis".JOURNAL OF SEPARATION SCIENCE 40.8(2017):1667-1673.
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