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First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-kappa B in Living Cells
Wang, Wanhe; Yang, Chao; Lin, Sheng; Vellaisamy, Kasipandi; Li, Guodong; Tan, Weihong; Leung, Chung-Hang; Ma, Dik-Lung
2017-04-06
Source PublicationCHEMISTRY-A EUROPEAN JOURNAL
ISSN0947-6539
Volume23Issue:20Pages:4929-4935
AbstractNF-kappa B is a critical transcription factor that plays an important role in mediating inflammation, the immune response, and cell proliferation. The activation of NF-kappa B leads to an enhancement of proinflammatory mediator expression, which is implicated in the pathogenesis of a variety of diseases. Therefore, methods that allow the intracellular tracking of NF-kappa B are particularly attractive because they can provide information regarding the pathways or stimulation responses that are involved in the activation of NF-kappa B. In this work, we report a novel platform to track intracellular NF-kappa B by employing the conjugated iridium(III) complex 1, which was synthesized through the unique combination of a luminescent iridium(III) moiety with the natural product oridonin. Experiments conducted with p50 knockdown cells revealed that complex 1 could detect the p50 subunit of NF-kappa B in cellulo. Furthermore, complex 1 tracked NF-kappa B translocation induced by tumor necrosis factor-alpha (TNF-alpha) as a model stimulus, without affecting the translocation process itself. To the best of our knowledge, complex 1 is the first metal-based compound that has been reported to be capable of monitoring intracellular NF-kappa B in living cells.
Keywordcell imaging iridium(III) complex natural products NF-kappa B
DOI10.1002/chem.201700770
URLView the original
Indexed BySCI ; IC
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000399327100026
PublisherWILEY-V C H VERLAG GMBH
The Source to ArticleWOS
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wang, Wanhe,Yang, Chao,Lin, Sheng,et al. First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-kappa B in Living Cells[J]. CHEMISTRY-A EUROPEAN JOURNAL,2017,23(20):4929-4935.
APA Wang, Wanhe.,Yang, Chao.,Lin, Sheng.,Vellaisamy, Kasipandi.,Li, Guodong.,...&Ma, Dik-Lung.(2017).First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-kappa B in Living Cells.CHEMISTRY-A EUROPEAN JOURNAL,23(20),4929-4935.
MLA Wang, Wanhe,et al."First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-kappa B in Living Cells".CHEMISTRY-A EUROPEAN JOURNAL 23.20(2017):4929-4935.
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