UM
Licocoumarone isolated from Glycyrrhiza uralensis selectively alters LPS-induced inflammatory responses in RAW 264.7 macrophages
Wu, Lehao; Fan, Yunpeng; Fan, Chao; Yu, Yang; Sun, Lei; Jin, Yu; Zhang, Yan; Ye, Richard D.
2017-04-15
Source PublicationEUROPEAN JOURNAL OF PHARMACOLOGY
ISSN0014-2999
Volume801Pages:46-53
AbstractThe effects of licocoumarone (LC) isolated from Glycyrrhiza uralensis were studied in LPS-stimulated RAW 264.7 macrophages. Our study demonstrated that LC dose-dependently attenuated LPS-induced NO production by down-regulating iNOS expression. Additionally, the treatment with LC inhibited LPS-induced expression of cytokines including IL-10, IL-6 and IL-10, but not TNF-alpha, at both mRNA and protein levels. Similar suppressive effects of LC were observed on LPS-stimulated murine peritoneal macrophages as well. Furthermore, LC significantly reduced LPS-stimulated NF-kappa B activation by inhibition of I kappa B alpha degradation and p65 phosphorylabon. The results from NF-kappa B-luc reporter gene assay further support the inhibitory effect of LC on NF-kappa B activation. Further studies showed that LC also interfered with the MAPKs and STAT3 signaling pathways, which are typical inflammatory signaling pathways triggered by LPS. Taken together, these results show that LC attenuates LPS-induced cytokine gene expression in RAW 264.7 macrophages through mechanisms that involve NF-kappa B, MAPKs and STAT3 signaling pathways, but the pattern of inhibition differs from that of a global immunosuppresant. Our study indicates that LC is a functional constituent of Glycyrrhiza uralensis with potential implications in infectious and immune-related diseases.
KeywordLicocoumarone Glycyrrhiza uralensis NF-kappa B MAPKs STAT3
DOI10.1016/j.ejphar.2017.02.049
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000399516400006
PublisherELSEVIER SCIENCE BV
The Source to ArticleWOS
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Wu, Lehao,Fan, Yunpeng,Fan, Chao,et al. Licocoumarone isolated from Glycyrrhiza uralensis selectively alters LPS-induced inflammatory responses in RAW 264.7 macrophages[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2017,801:46-53.
APA Wu, Lehao.,Fan, Yunpeng.,Fan, Chao.,Yu, Yang.,Sun, Lei.,...&Ye, Richard D..(2017).Licocoumarone isolated from Glycyrrhiza uralensis selectively alters LPS-induced inflammatory responses in RAW 264.7 macrophages.EUROPEAN JOURNAL OF PHARMACOLOGY,801,46-53.
MLA Wu, Lehao,et al."Licocoumarone isolated from Glycyrrhiza uralensis selectively alters LPS-induced inflammatory responses in RAW 264.7 macrophages".EUROPEAN JOURNAL OF PHARMACOLOGY 801(2017):46-53.
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