UM  > 科技學院
Identify the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in oil and aqueous phases of petroleum reservoirs with different temperatures from Chi
Zhichao Zhou1; Bo Liang2; Li-Ying Wang2; Jin-Feng Liu2; Bo-Zhong Mu2; Hojae Shim3; Ji-Dong Gu1
2019
Source PublicationBiogeosciences
ISSN1726-4170
Abstract

Microorganisms in petroleum reservoirs mediate hydrocarbon degradation coupling with methanogenesis process as the terminal step, they also contribute to microbial enhanced oil recovery (MEOR) worldwide with great economic and environmental benefits. Here, a molecular investigation, using the 16S rRNA and mcrA gene profiles based on MiSeq sequencing and clone library construction method, was conducted on oil and water (aqueous) phases of samples with high, moderate and low temperatures from seven petroleum reservoirs in China. A core bacterial microbiome with a small proportion of shared OUT, but a high proportion of sequences among all reservoirs was discovered, including aerobic degraders, sulfate/nitrate reducing bacteria, fermentative bacteria and sulfur cycling bacteria distributed mainly in Proteobacteria, Bacteroidetes, Deferribacteres, Deinococcus-Thermus, Firmicutes, Spirochaetae and Thermotogae. Their prevalence in the reported petroleum reservoirs and successive enrichment cultures suggest their common roles and functions involved in aliphatic and aromatic hydrocarbon degradation. Dominant methanogenesis process generally shifts from hydrogenotrophic pathway in water phase to acetoclastic pathway in oil phase in high temperature reservoirs, but the opposite is true for low temperature reservoirs. No differences were detected between two phases in moderate temperature reservoirs. Physiochemical factors, including pH, temperature, phase conditions, and nitrate, Mn2+, and Mg2+ concentrations are the main ones correlating to the microbial compositional and functional profiles significantly. LEfSe analysis shows distribution differences of microbial groups towards pH, temperature, and oil/water. Tax4Fun functional profiling indicates major functional metabolism differences between the two phases, including amino acids, hydrocarbons in the oil phase, and carbohydrates in the water phase.

KeywordReservoir Core Microbiome Oil And Water Phases Temperature Methanogenesis Hydrocrbon
DOIhttps://doi.org/10.5194/bg-2018-470
Language英语
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionFaculty of Science and Technology
DEPARTMENT OF CIVIL AND ENVIRONMENTAL ENGINEERING
Corresponding AuthorJi-Dong Gu
Affiliation1.Laboratory of Environmental Microbiology and Toxicology, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, Hong Kong, People’s Republic of China
2.State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, People’s Republic of China
3.Faculty of Science and Technology, University of Macau, Macau, People’s Republic of China
Recommended Citation
GB/T 7714
Zhichao Zhou,Bo Liang,Li-Ying Wang,et al. Identify the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in oil and aqueous phases of petroleum reservoirs with different temperatures from Chi[J]. Biogeosciences,2019.
APA Zhichao Zhou.,Bo Liang.,Li-Ying Wang.,Jin-Feng Liu.,Bo-Zhong Mu.,...&Ji-Dong Gu.(2019).Identify the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in oil and aqueous phases of petroleum reservoirs with different temperatures from Chi.Biogeosciences.
MLA Zhichao Zhou,et al."Identify the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in oil and aqueous phases of petroleum reservoirs with different temperatures from Chi".Biogeosciences (2019).
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhichao Zhou]'s Articles
[Bo Liang]'s Articles
[Li-Ying Wang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhichao Zhou]'s Articles
[Bo Liang]'s Articles
[Li-Ying Wang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhichao Zhou]'s Articles
[Bo Liang]'s Articles
[Li-Ying Wang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.