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Development of PCR and Real-time qPCR for Identifying and Quantifying Cyanobacteria
Weiying Zhang1; In Chio Lou1; Kai Meng Mok1; Wai Kin Ung2; Yijun Kong2
Conference NameInternational conference on engineering and applied science
Source PublicationProceedings of International Conference on Engineering and Applied Science
Conference DateJuly 24-27,2012
Conference PlaceBeijing, China
PublisherICEAS Publisher

In recent years, Macau Main Storage Reservoir (MSR) is experiencing a high level of cyanobacteria, particularly Microcystis spp. and C. raciborskii that can produce microcystin and cylindrospermposin, respectively. Long-time exposure to those cyanotoxins would affect the health of public, and thus reliable detection and quantification of the algae species are challenging in water quality management. The traditional manual counting is mainly involving microscopic identification and counting of cells, which are limited by inaccuracy and time-consuming. As development of molecular techniques and increasing number of microbial sequences available in the Genbank database, the use of molecular methods can be used for more rapid, reliable and accurate detection and quantification. In this study, polymerase chain reactor (PCR) and real-time quantitative PCR (qPCR) techniques were developed and optimized. The results showed that the techniques were successful for identifying and quantifying the species in pure cultures and mixed cultures, and were proved to be potential application to water samples in MSR. Similar cells numbers estimated by microscopic counting and qPCR were obtained, when the target species exceeded approximately 1 million cells/L. The molecular approaches provide another reliable monitoring option, in addition to the traditional microscopic counting, for drinking water monitoring program.

KeywordCyanobacteria C. raC.borskii Microscopy Pcr And Real-time qPcr Microcystis Spp.
URLView the original
Fulltext Access
Document TypeConference paper
Affiliation1.Faculty of Science and Technology, University of Macau, Macau SAR, China
2.Laboratory & Research Center, Macao Water Co. Ltd., Macau SAR, China
First Author AffilicationFaculty of Science and Technology
Recommended Citation
GB/T 7714
Weiying Zhang,In Chio Lou,Kai Meng Mok,et al. Development of PCR and Real-time qPCR for Identifying and Quantifying Cyanobacteria[C]:ICEAS Publisher,2012.
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