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Analysis of cylindrospermopsin- and microcystin-producing genotypes and cyanotoxin concentrations in the Macau storage reservoir
Weiying Zhang1; Inchio Lou1; Wai Kin Ung2; Yijun Kong2; Kai Meng Mok1
2014-12
Conference Name1st international conference on Challenges in Aquatic Sciences
Source PublicationHydrobiologia
Volume741
Issue1
Pages51–68
Conference DateMAR 15-21, 2013
Conference PlaceKeelung, TAIWAN
PublisherSPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Abstract

The Macau storage reservoir (MSR) has experienced algal blooms in recent years, with high levels of Cylindrospermopsis and Microcystis and detectable concentrations of cyanotoxins. To analyze the cyanotoxin-producing genotypes and relate the corresponding cyanotoxins to the water quality parameters, a quantitative real-time polymerase chain reaction was developed and applied to the water samples in three locations of MSR. Cylindrospermopsin polyketide synthetase (pks) gene and a series of microcystin synthetase (mcy) genes were used for identifying and quantifying cylindrospermopsin- and microcystin-producing genes, and the corresponding water parameters were measured accordingly. Our results showed that high concentrations of cylindrospermopsin and low concentrations of microcystin were measured during the study period. There was a strong correlation between the pks gene numbers and cylindrospermopsin concentrations (R2 = 0.95), while weak correlations were obtained between the mcy genes numbers and microcystin concentrations. Furthermore, the pks gene numbers were strongly related to Cylindrospermopsis (R2 = 0.88), cyanobacterial cell numbers (R2 = 0.96), total algae numbers (R2 = 0.95), and chlorophyll-a concentrations (R2 = 0.83), consistent with the dominant species of Cylindrospermopsis among the cyanobacteria existing in MSR. NH4–N (R2 = 0.68) and pH (R2 = 0.89) were the water quality parameters most highly correlated with the pks gene numbers. These results contribute to monitoring for potential cyanotoxins in raw water.

KeywordPks Cylindrospermopsin Microcystin Toxic Cyanobacteria Pcr And qPcr Mcy
DOIhttps://doi.org/10.1007/s10750-013-1776-2
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000343998000006
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeConference paper
CollectionDEPARTMENT OF CIVIL AND ENVIRONMENTAL ENGINEERING
Affiliation1.Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Av. Padre Toma´s Pereira Taipa, Macau, SAR, China
2.Laboratory & Research Center, Macao Water Co. Ltd., 718, Avenida do Conselheiro Borja, Macau, SAR, China
First Author AffilicationFaculty of Science and Technology
Recommended Citation
GB/T 7714
Weiying Zhang,Inchio Lou,Wai Kin Ung,et al. Analysis of cylindrospermopsin- and microcystin-producing genotypes and cyanotoxin concentrations in the Macau storage reservoir[C]:SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS,2014:51–68.
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