UM
Role of brain-derived neurotrophic factor and nerve growth factor in the regulation of Neuropeptide W in vitro and in vivo
Wang, Rikang; Yan, Fengxia; Liao, Rifang; Wan, Pei; Little, Peter J.; Zheng, Wenhua
2017-05-15
Source PublicationMOLECULAR AND CELLULAR ENDOCRINOLOGY
ISSN0303-7207
Volume447Issue:CPages:71-78
AbstractNerve growth factor (NGF) and Brain-derived neurotrophic factor (BDNF) are neurotrophic factors involved in the growth, survival and functioning of neurons. In addition, a possible role of neurotrophins, particularly BDNF, in HPA axis hyperactivation has recently been proposed. Neuropeptide W (NPW) is an endogenous peptide ligand for the GPR7 and GPR8 and a stress mediator in the hypothalamus. It activates the HPA axis by working on hypothalamic corticotrophin-releasing hormone (CRH). No information is available about the interrelationships between neurotrophines like NGF/BDNF and NPW. We studied the effect and underlying mechanisms of NGF/BDNF on the production of NPW in PC12 cells and hypothalamus. NGF time- and concentration-dependently stimulated the expression of NPW in PC12 cells. The effect of NGF was blocked by the inhibition of PI3K/Akt signal pathway with specific inhibitors for PI3K or AktsiRNA for Akt while inhibition of ERK pathway had no effect. Moreover, BDNF concentration dependently induced the expression of NPW mRNA and decreased the expression of NPY mRNA in primary cultured hypothalamic neurons which was also blocked by a PI3K kinase inhibitor. Finally, in vivo study showed that exogenous BDNF injected icy increased NPW production in the hypothalamus and this effect was reversed by a PI3 kinase inhibitor. These results and the fact that BDNF was able to stimulate the expression of CRH demonstrated that neurotrophines can modulate the expression of NPW in neuronal cells via the PI3K/Akt pathway and suggest that BDNF might be involved in functions of the HPA axis, at least in part by modulating the expression of NPW/NPY and CRH. (C) 2017 Elsevier B.V. All rights reserved.
KeywordNeuropeptide W Neurotrophins PI3K/Akt HPA axis
DOI10.1016/j.mce.2017.02.040
URLView the original
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Endocrinology & Metabolism
WOS SubjectCell Biology ; Endocrinology & Metabolism
WOS IDWOS:000400719300008
PublisherELSEVIER IRELAND LTD
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Wang, Rikang,Yan, Fengxia,Liao, Rifang,et al. Role of brain-derived neurotrophic factor and nerve growth factor in the regulation of Neuropeptide W in vitro and in vivo[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2017,447(C):71-78.
APA Wang, Rikang,Yan, Fengxia,Liao, Rifang,Wan, Pei,Little, Peter J.,&Zheng, Wenhua.(2017).Role of brain-derived neurotrophic factor and nerve growth factor in the regulation of Neuropeptide W in vitro and in vivo.MOLECULAR AND CELLULAR ENDOCRINOLOGY,447(C),71-78.
MLA Wang, Rikang,et al."Role of brain-derived neurotrophic factor and nerve growth factor in the regulation of Neuropeptide W in vitro and in vivo".MOLECULAR AND CELLULAR ENDOCRINOLOGY 447.C(2017):71-78.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Rikang]'s Articles
[Yan, Fengxia]'s Articles
[Liao, Rifang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Rikang]'s Articles
[Yan, Fengxia]'s Articles
[Liao, Rifang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Rikang]'s Articles
[Yan, Fengxia]'s Articles
[Liao, Rifang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.