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Cytotoxic and apoptosis-inducing activity of C-21 steroids from the roots of Cynanchum atratum
Zhang, Jian; Ma, Lin; Wu, Zheng-Feng; Yu, Shu-Le; Wang, Lei; Ye, Wen-Cai; Zhang, Qing-Wen; Yin, Zhi-Qi
2017-06
Source PublicationSTEROIDS
ISSN0039-128X
Volume122Pages:1-8
AbstractTwo new (1-2) and two known C-21 steroids (3-4) were isolated from the roots of Cynanchum atratum. Their structures were elucidated by detailed 1D and 2D spectroscopic. The MIT assay showed that compounds 1-4 displayed obvious cytotoxic activities against HepG2 cells with IC50 values ranging from 10.19 mu M to 76.12 mu M. Compounds 1-3 also exhibited cytotoxic effects in A549 cells with IC50 values of 30.87-95.39 mu M. Compound 3 showed the antiproliferative activity via GO/G1 cell cycle arrest and proapoptosis in HepG2 cells by Flowcytometry analysis. Western blotting analysis revealed that compound 3 could induce HepG2 cell apoptosis via the mitochondrial pathway by downregulating Bcl-2 expression, upregulating Bax protein expression, and activating caspase-9 and caspase-3. (C) 2017 Elsevier Inc. All rights reserved.
KeywordCynanchum atratum C-21 steroids Cytotoxic activity Apoptosis Mitochondrial pathway
DOI10.1016/j.steroids.2017.03.004
URLView the original
Indexed BySCI ; IC
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS SubjectBiochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS IDWOS:000401387200001
PublisherELSEVIER SCIENCE INC
The Source to ArticleWOS
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Zhang, Jian,Ma, Lin,Wu, Zheng-Feng,et al. Cytotoxic and apoptosis-inducing activity of C-21 steroids from the roots of Cynanchum atratum[J]. STEROIDS,2017,122:1-8.
APA Zhang, Jian.,Ma, Lin.,Wu, Zheng-Feng.,Yu, Shu-Le.,Wang, Lei.,...&Yin, Zhi-Qi.(2017).Cytotoxic and apoptosis-inducing activity of C-21 steroids from the roots of Cynanchum atratum.STEROIDS,122,1-8.
MLA Zhang, Jian,et al."Cytotoxic and apoptosis-inducing activity of C-21 steroids from the roots of Cynanchum atratum".STEROIDS 122(2017):1-8.
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