UM
Insight into the binding interaction of kaempferol-7-O-alpha-L-rhamnopyranoside with human serum albumin by multiple fluorescence spectroscopy and molecular modeling
Zhang, Wenting; Chen, Chun; Zhang, Chunping; Duan, Jingyu; Yao, Huankai; Li, Yan; Meng, Aiguo; Shi, Jun
2017-06
Source PublicationEXPERIMENTAL AND THERAPEUTIC MEDICINE
ISSN1792-0981
Volume13Issue:6Pages:3619-3623
AbstractHuman serum albumin (HSA) is a transporting protein that has multiple functions. The binding interaction between HSA and small molecules affects its function and efficacy of small molecules. The present study reports that kaempferol-7-O-alpha-L-rhamnopyranoside (KR) interacts with HSA as indicated by multiple fluorescence spectroscopy and molecular modeling. KR can quench the intrinsic fluorescence of HSA through the formation of a KR-HSA complex in a static manner. In addition, the binding site is located in subdomain IIA as confirmed by competitive experiments using site-specific warfarin and ibuprofen, and the driving forces include hydrogen bonds, van der Waals forces and electrostatic interaction derived from a thermodynamic analysis. The formation of KR-HSA is exothermic and spontaneous. Although there is no hydrophobic interaction around Tyr and Trp residues, the secondary structure of HSA changes through the formation of the KR-HSA complex. In addition, docking results visualized and further supported these results. Finally, these results can provide more information to further investigate the use of KR on the prevention of diabetic complications.
Keywordflavonoid human serum albumin binding interaction fluorescence spectroscopy molecular modeling
DOI10.3892/etm.2017.4427
URLView the original
Indexed BySCI
Language英语
WOS Research AreaResearch & Experimental Medicine
WOS SubjectMedicine, Research & Experimental
WOS IDWOS:000401080000150
PublisherSPANDIDOS PUBL LTD
The Source to ArticleWOS
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Zhang, Wenting,Chen, Chun,Zhang, Chunping,et al. Insight into the binding interaction of kaempferol-7-O-alpha-L-rhamnopyranoside with human serum albumin by multiple fluorescence spectroscopy and molecular modeling[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2017,13(6):3619-3623.
APA Zhang, Wenting.,Chen, Chun.,Zhang, Chunping.,Duan, Jingyu.,Yao, Huankai.,...&Shi, Jun.(2017).Insight into the binding interaction of kaempferol-7-O-alpha-L-rhamnopyranoside with human serum albumin by multiple fluorescence spectroscopy and molecular modeling.EXPERIMENTAL AND THERAPEUTIC MEDICINE,13(6),3619-3623.
MLA Zhang, Wenting,et al."Insight into the binding interaction of kaempferol-7-O-alpha-L-rhamnopyranoside with human serum albumin by multiple fluorescence spectroscopy and molecular modeling".EXPERIMENTAL AND THERAPEUTIC MEDICINE 13.6(2017):3619-3623.
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