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Simultaneous determination of components with wide polarity and content ranges in Cistanche tubulosa using serially coupled reverse phase-hydrophilic interaction chromatography-tandem mass spectrometry
Yan, Yu1,2; Song, Qingqing1; Chen, Xiaojia3; Li, Jun1; Li, Peng3; Wang, Yitao3; Liu, Tongxiang2; Song, Yuelin1; Tu, Pengfei1
2017-06-09
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1501Pages:39-50
Abstract

To meet the demands from plant physiologists and pharmacognosists, sustainable efforts are being devoted by the analytical chemists from all over the world to search an approach being capable of simultaneously monitoring primary along with secondary metabolites. The key technical bottlenecks currently lie at affording satisfactory chromatographic and spectrometric performances for both hydrophilic and hydrophobic substances that span a great content range. Herein, reverse phase liquid chromatography was directly coupled with hydrophilic interaction chromatography, namely RPLC-HILIC, to integrate their merits, whereas dilution pumps were employed to tackle the mismatching for the mobile phase between them. On the other side, inferior parameters rather than the optimal ones were applied for those abundant ingredients to advance the upper limits of quantitation, such as echinacoside (1250.0 mu g/mL), mannitol (100.0 mu g/mL), and acteoside (125 mu g/mL), in mass spectrometer domain. As a desert parasitic plant as well as tonic materials, Cistanche tubulosa (CT) has drawn widely interests from plant physiologists and pharmacognosists regarding its quantitative metabolome. Simultaneous determination of 23 abundant and minor ingredients covering most chemical families in CT, i.e. amino acid, nucleoside, organic acid, phenylethanoid glycoside, lignan, and iridoid, was attempted to understand the physiologic patterns as well as pharmacological values of the crude materials. Although the analytes spanned wide polarity (-3.326 <= cLogP <= 1.421) and content (more than 5 orders of magnitude) ranges, satisfactory chromatographic and spectrometric behaviors were gained for all analytes. Reliable quantitation was demonstrated via various method validation assays, such as recovery, linearity, sensitivity, and precision. The contents of 23 hydrophilic and hydrophobic substances were quantified in 20 batches of CT. Significant variations occurred for the contents of those components. Echinacoside (1.35-387.50 mg/g) was usually observed as the most abundant component, whereas ferulic acid (< 0.0043 mg/g) always exhibited trace distributions. Above all, the integrated equipment setup could serve as a fit-for-purpose tool for in-depth quality evaluation of CT and more importantly, for comprehensively understanding the metabolome of plants. (C) 2017 Elsevier B.V. All rights reserved.

KeywordCistanche Tubulosa Wide Polarity Span Upper Limit Of Quantitation Serially Coupled Reverse Phase-hydrophilic Interaction Chromatography Simultaneous Determination
DOIhttp://doi.org/10.1016/j.chroma.2017.04.034
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000402345100005
PublisherELSEVIER SCIENCE BV
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorSong, Yuelin; Tu, Pengfei
Affiliation1.Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
2.Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa 999078, Macao
Recommended Citation
GB/T 7714
Yan, Yu,Song, Qingqing,Chen, Xiaojia,et al. Simultaneous determination of components with wide polarity and content ranges in Cistanche tubulosa using serially coupled reverse phase-hydrophilic interaction chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1501:39-50.
APA Yan, Yu.,Song, Qingqing.,Chen, Xiaojia.,Li, Jun.,Li, Peng.,...&Tu, Pengfei.(2017).Simultaneous determination of components with wide polarity and content ranges in Cistanche tubulosa using serially coupled reverse phase-hydrophilic interaction chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1501,39-50.
MLA Yan, Yu,et al."Simultaneous determination of components with wide polarity and content ranges in Cistanche tubulosa using serially coupled reverse phase-hydrophilic interaction chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1501(2017):39-50.
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