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Digital microfluidic programmable stencil (dMPS) for protein and cell patterning
Gao Y.1; Tian J.1; Wu J.2; Cao W.1; Zhou B.3; Shen R.4; Wen W.1
2016
Source PublicationRSC Advances
ISSN20462069
Volume6Issue:104Pages:101760-101769
AbstractPatterning biomolecules and cells on substrates is usually a prerequisite for biological analysis and cell studies. A stencil is a versatile tool for sample patterning owing to its reusability and easy operation but it lacks addressable fluid control and programmable change of pattern. Here, we combined the advantages of a microfluidic chip and stencil to design a digitally controlled microfluidic programmable stencil. The key design is automatic passive matrix addressing based on combined application of two types of elastomeric valves. These two valves have distinct actuation thresholds, typically 13.4 psi for a round valve and much higher than 13.4 psi for a rectangle valve. Different types of protein and cell pattern on a 2D substrate could be obtained by controlling the fluid addressing code based on the passive matrix addressing method. An automatic microsampler was also applied to facilitate fast sample selection and introduction in the patterning process. A successful protein and cell pattern was obtained which could be used for downstream analysis and study.
DOI10.1039/c6ra17633j
URLView the original
Language英語
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被引频次[WOS]:3   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题University of Macau
Affiliation1.Hong Kong University of Science and Technology
2.Shanghai University
3.Universidade de Macau
4.Institute of Physics Chinese Academy of Sciences
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Gao Y.,Tian J.,Wu J.,et al. Digital microfluidic programmable stencil (dMPS) for protein and cell patterning[J]. RSC Advances,2016,6(104):101760-101769.
APA Gao Y..,Tian J..,Wu J..,Cao W..,Zhou B..,...&Wen W..(2016).Digital microfluidic programmable stencil (dMPS) for protein and cell patterning.RSC Advances,6(104),101760-101769.
MLA Gao Y.,et al."Digital microfluidic programmable stencil (dMPS) for protein and cell patterning".RSC Advances 6.104(2016):101760-101769.
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