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Functional Analysis of Nuclear Estrogen Receptors in Zebrafish Reproduction by Genome Editing Approach
Lu, Huijie1; Cui, Yong2; Jiang, Liwen2; Ge, Wei1
2017-07
Source PublicationENDOCRINOLOGY
ISSN0013-7227
Volume158Issue:7Pages:2292-2308
Abstract

Estrogens signal through both nuclear and membrane receptors with most reported effects being mediated via the nuclear estrogen receptors (nERs). Although much work has been reported on nERs in the zebrafish, there is a lack of direct genetic evidence for their functional roles and importance in reproduction. To address this issue, we undertook this study to disrupt all three nERs in the zebrafish, namely esr1 (ER alpha), esr2a (ER beta II), and esr2b (ER beta I), by the genome-editing technology clustered regularly interspaced short palindromic repeats and its associated nuclease (CRISPR/Cas9). Using this loss-of-function genetic approach, we successfully created three mutant zebrafish lines with each nER knocked out. In addition, we also generated all possible double and triple knockouts of the three nERs. The phenotypes of these mutants in reproduction were analyzed in all single, double, and triple nER knockouts in both females and males. Surprisingly, all three single nER mutant fish lines display normal reproductive development and function in both females and males, suggesting functional redundancy among these nERs. Further analysis of double and triple knockouts showed that nERs, especially Esr2a and Esr2b, were essential for female reproduction, and loss of these two nERs led to an arrest of folliculogenesis at previtellogenic stage II followed by sex reversal from female to male. In addition, the current study also revealed a unique role for Esr2a in follicle cell proliferation and transdifferentiation, follicle growth, and chorion formation. Taken together, this study provides the most comprehensive genetic analysis for differential functions of esr1, esr2a, and esr2b in fish reproduction.

DOI10.1210/en.2017-00215
URLView the original
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000405105100031
PublisherOXFORD UNIV PRESS INC
The Source to ArticleWOS
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Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Centre of Reproduction, Development, and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau
2.School of Life Sciences, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, Hong Kong
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Lu, Huijie,Cui, Yong,Jiang, Liwen,et al. Functional Analysis of Nuclear Estrogen Receptors in Zebrafish Reproduction by Genome Editing Approach[J]. ENDOCRINOLOGY,2017,158(7):2292-2308.
APA Lu, Huijie,Cui, Yong,Jiang, Liwen,&Ge, Wei.(2017).Functional Analysis of Nuclear Estrogen Receptors in Zebrafish Reproduction by Genome Editing Approach.ENDOCRINOLOGY,158(7),2292-2308.
MLA Lu, Huijie,et al."Functional Analysis of Nuclear Estrogen Receptors in Zebrafish Reproduction by Genome Editing Approach".ENDOCRINOLOGY 158.7(2017):2292-2308.
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