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Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution
Dong, Zhen-Zhen1; Lu, Lihua1,2; Wang, Wanhe1; Li, Guodong3; Kang, Tian-Shu3; Han, Quanbin4; Leung, Chung-Hang3; Ma, Dik-Lung1,5
2017-07
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume246Pages:826-832
Abstract

A cyclometalated iridium(III) complex has been synthesized as a novel luminescent probe for G-quadruplex (G4) DNA in aqueous solution, and a G4-based assay was employed for the detection of nicking endonuclease activity using Nb.Bsml as a model enzyme. In the assay, an initial hairpin DNA (5'-TG(4)AG(3)TG(4)AG(3)TG(4)A(2)G(3)A(2)TGCTA(3)TAGCAT(2)C(3)T(2)C(4)AC-3') sequence containing the Nb.Bsml recognition site in its stem region and a partially caged G-rich DNA sequence at its 5'-terminus could be cleaved by Nb.BsmI, which liberates the G-rich DNA sequence and enhances the luminescence of complex 1. This assay showed a linear relation between luminescent signal and Nb.Bsml concentration over the range of 0-10 U/mL (R-2=0.9937) with a detection limit for Nb.Bsml of 0.042 U/mL. Furthermore, this assay showed high selectivity for Nb.Bsmi over other nicking endonucleases, and functioned effectively in the presence of cell lysates. (C) 2017 Elsevier B.V. All rights reserved.

KeywordIridium(Iii) Complex Nicking Endonuclease Luminescence G-quadruplex Probe
DOI10.1016/j.snb.2017.02.156
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000400803700101
PublisherELSEVIER SCIENCE SA
The Source to ArticleWOS
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Co-First AuthorDong, Zhen-Zhen
Corresponding AuthorLeung, Chung-Hang; Ma, Dik-Lung
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
4.School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
5.Institute of Research and Continuing Education, Hong Kong Baptist Unversity, Shenzhen 518057, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Dong, Zhen-Zhen,Lu, Lihua,Wang, Wanhe,et al. Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,246:826-832.
APA Dong, Zhen-Zhen.,Lu, Lihua.,Wang, Wanhe.,Li, Guodong.,Kang, Tian-Shu.,...&Ma, Dik-Lung.(2017).Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution.SENSORS AND ACTUATORS B-CHEMICAL,246,826-832.
MLA Dong, Zhen-Zhen,et al."Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution".SENSORS AND ACTUATORS B-CHEMICAL 246(2017):826-832.
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