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A macrophage-activating, injectable hydrogel to sequester endogenous growth factors for in situ angiogenesis
Feng, Yanxian1; Li, Qiu1; Wu, Dang2; Niu, Yiming1; Yang, Cheng2; Dong, Lei3; Wang, Chunming1
2017-07
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume134Pages:128-142
Abstract

Biomaterials scaffolds designed for many regenerative applications are expected to support neovascularisation, which is now being hampered by two limitations - the instability of exogenous growth factors (GFs) that are delivered to promote angiogenesis; and the loss of extracellular matrix components that bind and stabilise GFs. Here, we report the design and evaluation of an injectable hydrogel system aimed at restoring a GF-binding microenvironment to enhance the pro-angiogenic functions of endogenous GFs. This gel comprises two polysaccharides with their unique bioactivities: Konjac glucomannan (KGM) as the building block of the gel scaffold, for its demonstrated capacity to activate macrophages/monocytes to secrete pro-angiogenic/-mitogenic GFs; and heparin (Hep), a representative glycosaminoglycan molecule that binds numerous pro-angiogenic GFs, as functional moieties to sequester the macrophage-produced GFs. Modified with tyramine (TA) groups, the two polysaccharides can be co-polymerised and rapidly form into hydrogel upon enzyme catalysis. The designed KGM-TA/Hep-TA hydrogel successfully preserves the macrophage-activating function and GF-binding affinity of the two components, respectively, and, once subcutaneously implanted, effectively sequestered the locally-produced GFs in situ and promote the formation and maturation of blood vessels in mice. In summary, the designed hydrogel system demonstrates a feasible approach to stimulate the production and harness the function of endogenous GFs for inducing blood vessel formation in vivo, without the addition of any exogenous proteins. This design may provide an innovative, open platform to promote vascularisation for various regenerative purposes. (C) 2017 Elsevier Ltd. All rights reserved.

KeywordAngiogenesis Macrophages Growth Factors Hydrogels Glycosaminoglycans Glucomannan
DOI10.1016/j.biomaterials.2017.04.042
URLView the original
Indexed BySCI
Language英语
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, bioMaterials
WOS IDWOS:000401717500011
PublisherELSEVIER SCI LTD
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorDong, Lei; Wang, Chunming
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau SAR, China
2.Division of Energy and Environment, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
3.State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Feng, Yanxian,Li, Qiu,Wu, Dang,et al. A macrophage-activating, injectable hydrogel to sequester endogenous growth factors for in situ angiogenesis[J]. BIOMATERIALS,2017,134:128-142.
APA Feng, Yanxian.,Li, Qiu.,Wu, Dang.,Niu, Yiming.,Yang, Cheng.,...&Wang, Chunming.(2017).A macrophage-activating, injectable hydrogel to sequester endogenous growth factors for in situ angiogenesis.BIOMATERIALS,134,128-142.
MLA Feng, Yanxian,et al."A macrophage-activating, injectable hydrogel to sequester endogenous growth factors for in situ angiogenesis".BIOMATERIALS 134(2017):128-142.
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