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Ratiometric fluorescent nanosensors for selective detecting cysteine with upconversion luminescence
Guan Y.2; Qu S.2; Li B.2; Zhang L.2; Ma H.2; Zhang L.2
2016-03-15
Source PublicationBiosensors and Bioelectronics
ISSN18734235 09565663
Volume77Pages:124-130
AbstractFluorescent sensors based on upconversion (UC) luminescence have been considered as a promising strategy to detect bio-analyte due to their advantages in deep penetration, minimum autofluorescence, and ratiometric fluorescent output. A prototype of nanosensors combined with mesoporous silica coated upconversion nanoparticles (UCNPs) and a fluorescein-based fluorescent probe loaded in pores was therefore designed to detect cysteine (Cys). The silica shell provided loading space for the probe and enabled the nanosensors to disperse in water. In the presence of Cys, the fluorescent probe was transformed into 5(6)-carboxyfluorescein with an emission band centering at 518nm which was secondarily excited by the light at around 475nm from NaYF4:Yb, Tm UCNPs driven by 980nm near-infrared (NIR) laser. The intensity ratio between green and blue luminescence (I/I) grew exponentially with increasing concentrations of Cys over a range of 20-200μmolL. The response of the nanosensors towards Cys was recognizable with naked eyes by luminescence color change. Evidences suggest that these nanosensors are capable of sensing Cys in aqueous solution and distinguishing Cys from homocysteine (Hcy) with kinetically-controlled selectivity. The system was further employed to detect Cys in human serum and the result was in agreement with it tested by high performance liquid chromatography with acceptable recovery.
KeywordCysteine Fluorescence Mesoporous silica Nanosensor Upconversion
DOI10.1016/j.bios.2015.09.034
URLView the original
Language英語
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Cited Times [WOS]:36   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.University of Chinese Academy of Sciences
2.Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences
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Guan Y.,Qu S.,Li B.,et al. Ratiometric fluorescent nanosensors for selective detecting cysteine with upconversion luminescence[J]. Biosensors and Bioelectronics,2016,77:124-130.
APA Guan Y.,Qu S.,Li B.,Zhang L.,Ma H.,&Zhang L..(2016).Ratiometric fluorescent nanosensors for selective detecting cysteine with upconversion luminescence.Biosensors and Bioelectronics,77,124-130.
MLA Guan Y.,et al."Ratiometric fluorescent nanosensors for selective detecting cysteine with upconversion luminescence".Biosensors and Bioelectronics 77(2016):124-130.
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