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Genome guided investigation of antibiotics producing actinomycetales strain isolated from a Macau mangrove ecosystem
Hu, Dini1; Chen, Yan2; Sun, Chenghang3; Jin, Tao4; Fan, Guangyi4; Liao, Qiwen2; Mok, Kai Meng1; Lee, Ming-Yuen Simon2
2018-09-24
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume8
Other Abstract

Actinomycetes are a heterogeneous group of gram positive filamentous bacteria that have been found to produce a wide range of valuable bioactive secondary metabolites, particularly antibiotics. Moreover, actinomycetes isolated from unexplored environments show an unprecedented potential to generate novel active compounds. Hence, in order to search for novel antibiotics, we isolated and characterized actinomycetes strains from plant samples collected from a mangrove in Macau. Within the class of actinobacteria, fourteen actinomycetes isolates have been isolated and identified belonging to the genus of Streptomyces, Micromonospora, Mycobacterium, Brevibacterium, Curtobacterium and Kineococcus based on their 16S rRNA sequences. Further whole genome sequencing analysis of one of the isolated Streptomyces sp., which presented 99.13% sequence similarity with Streptomyces parvulus strain 2297, showed that it consisted of 118 scaffolds, 8,348,559 base pairs and had a 72.28% G + C content. In addition, genome-mining revealed that the isolated Streptomyces sp. contains 109 gene clusters responsible for the biosynthesis of known and/or novel secondary metabolites, including different types of terpene, T1pks, T2pks, T3pks, Nrps, indole, siderophore, bacteriocin, thiopeptide, phosphonate, lanthipeptide, ectoine, butyrolactone, T3pks-Nrps, and T1pks-Nrps. Meanwhile, the small molecules present in ethyl acetate extract of the fermentation broth of this strain were analyzed by LC-MS. Predicted secondary metabolites of melanin and desferrioxamine B were identified and both of them were firstly found to be produced by the Streptomyces parvulus strain. Our study highlights that combining genome mining is an efficient method to detect potentially promising natural products from mangrove-derived actinomycetes.

DOIhttps://doi.org/10.1038/s41598-018-32076-z
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000445336600039
PublisherNATURE PUBLISHING GROUP
The Source to ArticleWOS
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF CIVIL AND ENVIRONMENTAL ENGINEERING
Corresponding AuthorLee, Ming-Yuen Simon
Affiliation1.Faculty of Science and Technology, Department of Civil and Environmental Engineering, University of Macau, Macao, China.
2.State Key Laboratory of Quality Research in Chinese Medicine and institute of Chinese Medical Sciences, University of Macau, Macao, China.
3.Institute of Medicinal Biotechnology, Chinese Academy of Medical Science & Peking Union Medical College, Tiantanxili No 1, Beijing, 100050, P.R. China.
4.Beijing Genome Institute– Shenzhen, Shenzhen, 518083, China.
First Author AffilicationFaculty of Science and Technology
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Hu, Dini,Chen, Yan,Sun, Chenghang,et al. Genome guided investigation of antibiotics producing actinomycetales strain isolated from a Macau mangrove ecosystem[J]. SCIENTIFIC REPORTS,2018,8.
APA Hu, Dini.,Chen, Yan.,Sun, Chenghang.,Jin, Tao.,Fan, Guangyi.,...&Lee, Ming-Yuen Simon.(2018).Genome guided investigation of antibiotics producing actinomycetales strain isolated from a Macau mangrove ecosystem.SCIENTIFIC REPORTS,8.
MLA Hu, Dini,et al."Genome guided investigation of antibiotics producing actinomycetales strain isolated from a Macau mangrove ecosystem".SCIENTIFIC REPORTS 8(2018).
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