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Artemisinin DamageProtects Retinal Neuronal Cells against Oxidative Stress and Restores Rat Retinal Physiological Function from Light Exposed
Yan, Fengxia; Wang, Haitao; Gao, Yang; Xu, Jiangping; Zheng, Wenhua
2017-08
Source PublicationACS CHEMICAL NEUROSCIENCE
ISSN1948-7193
Volume8Issue:8Pages:1713-1723
AbstractOxidative stress plays a key role in the pathogenesis of age-related macular degeneration (AMD), a leading cause of severe visual loss and blindness in the aging population which lacks any effective treatments currently. In this study, artemisinin, a well-known antimalarial drug was found to suppress hydrogen peroxide (H2O2)-induced cell death in retinal neuronal RGC-5 cells. Artemisinin, in the therapeutically relevant dosage, concentration-dependently attenuated the accumulation of intracellular reactive oxygen species (ROS), increased mitochondrial membrane potential and decreased cell apoptosis in RGC-5 cells induced by H2O2. Western blot analysis showed that artemisinin upregulated the phosphorylation of p38 and extracellular signal-regulated kinasesl/2 (ERK1/2) and reversed the inhibitory effect of H2O2 on the phosphorylation of these two kinases. Moreover, protective effect of artemisinin was blocked by the p38 kinase inhibitor PD169316 or ERK1/2 kinase pathway inhibitor PD98059, respectively. In contrast, c-Jun N-terminal kinase inhibitor and rapamycin had no effect in the protective effect of artemisinin. Taken together, these results demonstrated that artemisinin promoted the survival of RGC-5 cells from H2O2 toxicity via the activation of the p38 and ERK1/2 pathways. Interestingly, intravitreous injection of artimisinin, concentration-dependently reversed light exposed-damage (a dry AMD animal model) of rat retinal physiological function detected by flash electroretinogram. These results indicate that artemisinin can protect retinal neuronal functions from H2O2-induced damage in vitro and in vivo and suggest the potential application of artemisinin as a new drug in the treatment of retinal disorders like AMD.
KeywordAMD artemisin H2O2 ROS p38 ERK1/2
DOI10.1021/acschemneuro.7b00021
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Neurosciences & Neurology
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Medicinal ; Neurosciences
WOS IDWOS:000408077700016
PublisherAMER CHEMICAL SOC
The Source to ArticleWOS
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Yan, Fengxia,Wang, Haitao,Gao, Yang,et al. Artemisinin DamageProtects Retinal Neuronal Cells against Oxidative Stress and Restores Rat Retinal Physiological Function from Light Exposed[J]. ACS CHEMICAL NEUROSCIENCE,2017,8(8):1713-1723.
APA Yan, Fengxia,Wang, Haitao,Gao, Yang,Xu, Jiangping,&Zheng, Wenhua.(2017).Artemisinin DamageProtects Retinal Neuronal Cells against Oxidative Stress and Restores Rat Retinal Physiological Function from Light Exposed.ACS CHEMICAL NEUROSCIENCE,8(8),1713-1723.
MLA Yan, Fengxia,et al."Artemisinin DamageProtects Retinal Neuronal Cells against Oxidative Stress and Restores Rat Retinal Physiological Function from Light Exposed".ACS CHEMICAL NEUROSCIENCE 8.8(2017):1713-1723.
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